Clostridium histolyticum

Collagenase 400 U/ml collagenase from Clostridium histolyticum (high-... 

This enzyme [EC 3.4.22.8], also referred to as clostridio-peptidase B, is an endopeptidase belonging to the peptidase family Cll that has been isolated from the bacterium Clostridium histolyticum. It catalyzes the hydrolysis of peptide bonds with a preference for the Arg—Xaa bond (including the Arg—Pro bond), but not the Lys— Xaa peptide bond. This calcium ion-activated enzyme is inactivated by EDTA. 

Among cysteine proteases of bacteria is a papainlike enzyme from Clostridium histolyticum with a specificity similar to that of trypsin 338 Tire anaerobic Porphyromonas gingivalis, which is implicated in perio-dental disease, produces both arginine- and lysine-specific cysteine proteases designated gingipains.339 3393 Some virally encoded cysteine proteases, including one from the polio virus, have trypsin-like sequences with the serine of the catalytic triad replaced by cysteine.340 341 A human adenovirus protease also has a Cys His Glu triad but a totally different protein fold.342... 

Some years ago, collagenase from the microorganisms Clostridium histolyticum was shown to be a zinc metalloenzyme 1303 mammalian collagenases are also zinc metalloenzymes.1304 The collagenase-like peptidase of rat testis1305 has also been shown independently to be a zinc metalloenzyme. 

Dipfestion buffer 2 for osteoblast-like cells. Add 200 U/mL of type 1 collagenase from Clostridium histolyticum (Sigma) and 14.7 mg CaCl2 H20 (1 mM) in 100 mL HESS buffer ( ee Notes 1 and 2). 

Endoproteinase Arg-C Origin Clostridium histolyticum Roche Diagnostics Endoproteinase Arg-C, Sequencing Grade... 

Clostridium histolyticum collagenase. Clostridiopeptidase A. Digestion of native collagen in the triple helical region at Xaa-I-Gly... 

The increase in cell production due to UF is shown in Figure 3.103.76 Clostridium histolyticum is cultivated in batch and continuous fermentors. In the batch system, the organism approaches a stationary phase at about the twelfth hour due to toxic metabolite production. In the continuous fermentor, fresh substrate is fed continuously and the toxic products are continuously removed enabling growth to continue well past the fortieth hour. Likewise, the enzyme production from this fermentor is enhanced (see Figure... 

Islet isolation methods that yield islets free of contaminating fibroblasts now have been developed. Successful prevention of the growth of dense fibrous tissue on extravascular chambers containing these purified islets would raise new possibilities in the design of islet-transplantation chambers. This chapter contains the initial results of binding enzymes to Millipore AA cellulose ester membranes for application in such chambers. The enzyme chosen for study was collagenase from Clostridium histolyticum (Worthington Biochemicals, Inc.). 

The enzyme collagenase is derived from fermentation of Clostridium histolyticum. 

Clostripain [9028-00-6] Mr -55,000 [EC 3.4.22.8]. Clostripain is isolated from Clostridium histolyticum callogenase by extraction in pH 6.7 buffer, followed by hydroxylapatite chromatography with a 0.1-0.2 M phosphate gradient, then Sephadex G-75 gel filtration with 0.05M phosphate pH 6.7, dialysis and a second... 

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