Collagen solubility

Desamidocoiiagen K 1.0, Desamidocoiiagen K 1.5. See Soluble collagen Desamidocoiiagen. See Desamido collagen Soluble collagen Desamina. See Glucamine... 

Soluble Native Collagen RS-1 No. 26205, Soluble animal collagen. See Soluble collagen Soluble blue. See Acid blue 93 Soluble collagen... 

Synonyms Soluble animal collagen Soluble native collagen... 

Synonyms Soluble animal collagen Soluble native collagen Definition Nonhydrolyzed, native protein derived from connective tissue of young animals consists of a mixt. of precursors of mature collagen Properties M.w. 285,000... 

Neutral salt-soluble collagen as well as add-soluble collagen show a CD spectrum (Fig. 8) having bands at 198 nm, 0 = -53 (MX) (deg cm2 dmol-1), and at 223 nm, 0 = 7500 (deg cm2 dmol-1), the ratio between both being 7 1. Pysh has calculated the CD spectra of a-helix, -structure, polyproline I and II127 ... 

Fig. 37. Dependence of the thermodynamic parameters AH and AS of triple-helix formation on the imino acid content of the peptides (obtained by cleavage of calf skin-type I collagene with cyanogene and subequent isolation by column chromatrography)3) and of the native neutral salt-soluble skin collagene of various animals. The entropy values are denoted by dotted lines...

The diversity in primary, secondary, tertiary, and quaternary stmctures of proteins means that few generalisations can be made concerning their chemical properties. Some fulfil stmctural roles, such as the collagens (found in bone) and keratin (found in claws and beaks), and are insoluble in all solvents. Others, such as albumins or globulins of plasma, are very soluble in water. Still others, which form part of membranes of cells, are partly hydrophilic ( water-loving , hence water-soluble) and partly lipophilic ( lipid-loving , hence fat-soluble). 

Bone protein was extracted following the method described by Sealy (1986). Bone chips were demineralized in a weak HCl solution, then soaked in 0.1 M NaOH to remove base-soluble humic substances. Remaining material, which is mainly collagen, but includes non-collagenous proteins, was... 

Figure 9.2. Amino acid content (mol %) in collagen extract (dark columns) and serum proteins (light columns) from a skeleton from coastal Peru. Due to a prevalence of degraded and soluble collagen, the nonmineral-bound protein fraction shows a collagen amino acid profile.

Meyer M., Morgenstern B. 2003. Characterization of Gelatine and Acid Soluble Collagen by Size Exclusion Chromatography Coupled with Multi Angle Light Scattering (SEC-MALS). Biomacromolecules 4,1727-1732. 

Recently, water-soluble protein fractions, isolated from extracts of bone matrix, were incorporated into a collagen matrix and shown to induce bone (67,68) and cartilage formation both in vitro and in vivo (69,70). In the latter studies, in the absence of the collajgen delivery system, the proteins were incapable of inducing cartilage formation in vivo when implanted intramuscularly into mice. The success of this approach appears to depend on delivering the active agents at an effective dose over an extended time period. 

The human lens is rich in ascorbate, which is required for normal collagen synthesis and acts as a water-soluble antioxidant, reacting rapidly with superoxide, hydroxyl and peroxyl radicals. However, ascorbic acid can undergo auto-oxidation and, at certain concentrations, can form hydroxyl radicals with hydrogen peroxide in the presence of light and riboflavin as described above (Delaye and Tardieu, 1983 Ueno et al., 1987). 

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