Collagen physicochemical properties

Li CQ et al (2009) Construction of collagen II/hyaluronate/chondroitin-6-sulfate tri-copoly-mer scaffold for nucleus pulposus tissue engineering and preliminary analysis of its physicochemical properties and biocompatibility. J Mater Sci Mater Med 21 741-751... 

Nomura, Y., Toki, S., Ishii, Y., and Shirai, K. (2000a). The physicochemical property of shark type I collagen gel and membrane. J. Agric. Food Chem. 48,2028-2032. 

Figure 19.2 shows the different physicochemical properties of the PES membranes modified with RGD and galadose moiety with resped to the collagen that was used as a natural substrate. Native PES membranes have a very high hydrophilic surface charader, in fad, the water contad angle measured on this membrane was 30 1.4°. Also, the modified membranes display a marked wettability even if, in this case, the... 

The dissociation of the polypeptide chains of collagen by thermal or chemical processes leads to products variously termed gelatin. In the present section we propose to consider certain of the physicochemical properties of gelatin, looking toward a deeper understanding of the structure and chemistry of collagen. 

In one study on wound-covering materials with controlled physicochemical properties, an artificial skin was designed with a crosslinfced collagen-polysaccharide (chondroitin 6-sulfate) composite membrane. This was specifically chosen to have controlled porosity (5 to 150 /rm in diameter), flexibihty (by varying crosslink density), and moisture flux rate. 

Z. Zhang, G. Li, B. Shi, Physicochemical properties of collagen, gelatin and collagen hydrolysate derived from bovine limed split wastes, J. Soc. Leather Technol. Chem. 90 (2012) 23-28. 

Fibroblasts are the main cells of connective tissue responsible for the production of collagen and mucopolysaccharides. Physicochemical properties of surfaces, along with polymer matrix components, cell membranes, and cyto-skeleton, most hkely are involved in fibroblast adhesion [26]. Mirzadeh et al. [27] carried out in-vitro studies on the effect of crosshnking density on fibroblast cell behavior using mouse fibroblast L-929 cells. Their results showed that the crosslinks density, and consequently molecular mobility, cause changes to the cell behavior. The above researchers found that there is an optimum molecular mobility for PDMS at which the number and surface area of cells show the best cell attachment and proliferation. 

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