Cold Homogenization

The primary product of the hot homogenization is a nanoemulsion resulting from the liquid state of the lipid. Solid particles are expected to be formed by the cooling of the sample to room temperature or below. Because of the small particle size and the presence of the emulsifiers, lipid crystallization may be highly retarded, and the sample may remain as a supercooled melt (nanoemulsion) for several months [28], Westesen and Bunjes found that purported SLN data published by another group were, in fact, measurements from supercooled melts [29], 

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FIGURE 7.1 Atomic force microscopy image of prednisolone-loaded Compritol nanoparticles produced by cold homogenization. Imaging was performed by using the noncontact mode. The formulation is composed of 5% Compritol, 1% prednisolone, 2.5% poloxamer 188, and 92.5% water. (From zur Miihlen, A. and Mehnert, W., Pharmazie, 53, 552-55, 1998. With permission.)... 

Suspend the cells in cold homogenization buffer at 2 x 108 cells/mL. 

Frolov I wonder how you are going to apply cold homogeneous flow measurements to understand the dynamics of reactive heterogeneous flow in realistic combustors I anticipate this will be not a simple task as the flows I mentioned differ considerably. 

Remove the brains and transfer them to ice-cold homogenization buffer. 

Dissect the hippocampi on a cooled Petri dish and transfer them immediately to a 5-mL Teflon-glass homogenizer containing ice-cold homogenization buffer (2.5 mL of buffer/rat see Note 6). 

The homogenization process can be carried out at a temperature above the melting point of the lipid (melt homogenization) or at a temperature below the melting point of the lipid (cold homogenization). 

Formation of a zone with concentrations required for the intermediate phase resulting from the processes of such a type as cold homogenization or diffusion-induced grain boundary migration (DIGM). 

A stirred cold homogeneous mixture of thiophthalide and anhydrous ether treated under Ng during 5 min. with a soln. of vinyllithium in tetrahydrofuran from a syringe, warmed to room temp., then treated with acetic acid 3,4-dihydro-2-benzothiepin-5(lH)-one. Y 51%. F. e. and limitations s. W. C. Lumma, Jr., G. A. Dutra, and C. A. Voeker, J. Org. Chem. 35, 3442 (1970). 

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