Clostridium propionicum

In combination of this polymerase with purified propionyl-CoA transferase of Clostridium propionicum, a two-enzyme in vitro PHB biosynthesis system was established which allowed the PHB synthesis from (R)-hydroxybutyric acid as substrate [119]. In this way, the PHB synthesis was independent of the consumption of the expensive CoA, and hence PHA could be readily produced in a semipreparative-scale... 

Clostridium propionicum can use alanine as substrate for a balanced fermentation to form ammonium propionate, acetate, and C02 (Fig. 24-19). 

Figure 24-19 Fermentation of L-alanine by Clostridium propionicum.. After Buckel.363...

Fig. 5.21. The end-products (circled) of microbial fermentations of pyruvate. Letters indicate the organisms able to perform these reactions. (/<) Lactic acid bacteria (Streptococcus, Lactobacillus) (B) Clostridium propionicum (C) Yeast, Zymomonas mobilis, Sarcina ventriculr, (D) Enterobacteriaceae (Coli-aerogenes) (E) Clostridia, ...

Acrylic acid iS-Alanine Propionate Lactic acid Megasphaera elsdenii Clostridium propionicum Literature cited... 

Herrmann G, Selmer T, Jessen HJ, Gokarn RR, Selifonova O, Gort SJ, Buckel W. (2005). Two beta-alanyl-CoA ammonia lyases in Clostridium propionicum. FEBS J, 272, 813-821. 

Acrylic acid Rhodococcus rhodochrous Rhodococcus AJ270 Clostridium propionicum... 

A direct oxidation of the 3-carbon chain has been observed with extracts of Clostridium propionicum. The thioester propionyl pantetheine was used as a more available substrate than propionyl CoA, and... 

Rhodopseudomonas spheroides possible role of heme in regulation of the branched biosynthetic pathway. J Bacteriol 98 712-720 Laubinger W and Dimroth P (1987) Characterization of the Na -stimulated ATPase of Propionigenium modestum as an enzyme of the FiFq type. Eur J Biochem 168 475-480 Leaver FW, Wood HG and Stjemholm R (1955) The fermentation of three carbon substrates by Clostridium propionicum md Propionibacterium, J Bacteriol 70 521-530 Lee PC, Bochner BR and Ames BN (1983a) AppppA, heat shock stress, and cell oxidation. Proc Natl Acad Sci USA 80 7496-7500... 

Another highly potential production species is the gram-positive bacteria Clostridium propionicum. This microorganism is able to utilise lactate, glycerol and alanine as substrate. Propionate, acetate, formate, n-propanol and succinate are produced. The optimal pH value is 6.8 and the best temperature is 30 °C. C. propionicum uses the acrylic acid pathway to produce the desired product... 

Clostridium propionicum Cardon and Barker (1946), Ludwig et al. (2009), Janssen (1991)... 

O Brien, D.J., Panzer, C.C., Eisele, W.P., 1990. Biological production of acrylic acid from cheese whey by resting cells of Clostridium propionicum. Biotechnology Progress 6,237-242. 

Schweiger, G., Buckel, W., 1985. Identification of acrylate, the product of the dehydration of (R)-lactate catalyzed by cell-free extracts from Clostridium propionicum. FEES Letters 185,253-256. 

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