Clostridium cellulovorans

Murashima, K., Chen, C.-L., Kosugi, A. et al. (2002) Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes. Journal of Bacteriology, 184 (1), 76-81. 

Cho HY, Yukawa H, Inui M, Doi RH, Wong SL. (2004). Production of mini cellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl Environ Microbiol, 70, 5704-5707. 

Jeon E, Hyeon JE, Eun LS, Park BS, Kim SW, Lee J, Han SO. (2009). Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera p-glucosidase. FEMS Microbiol Lett, 301, 130-136. 

Recently, a fusion protein between a cellulose binding domain (CBOeios)> isolated from Clostridium cellulovorans, and OPH was shown to be capable of immobilization onto various cellulose materials (24). The use of cellulose as an immobilization matrix is advantageous due to its low cost, wide spread availability and non-toxic nature. The kinetic parameters of OPH fused to die CBD domain were essentially identical to die soluble protein with a 3.8 fold increase in from 0.058 to 0.220 and a 10.4% decrease in Kd from 3170 to 2840. Additionally, the immobilized fusion protein offered superior stability over that of soluble OPH, retaining over 85% activity over a period of 45 days (24). 

Shoseyov O, Doi RH (1990) Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase. Proc Natl Acad Sci USA 87 2192-2195 Simankova MV, Chemych NA, Osipov GA, Zavarzin GA (1993) Halocella cellulolytica, gen. nov. sp. nov., a new obligately anaerobic, halophilic, cellulolytic bacterium. Syst Appl... 

Goldstein, MA Takagi, M Hashida, S Shoseyov, 0 Doi, RH Segel, IH. Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein k. J Bacteriol, 1993, 175(18), 5762-8. 

Xu, Y Foong, EC. Characterization of a cellulose binding domain from Clostridium cellulovorans endoglucanase-xylanase D and its use as a fusion partner for soluble protein expression in Escherichia coli. J Biotechnol, 2008, 135(4), 319-25. 

Murashima, K Kosugi, A Doi, RH. Solubilization of cellulosomal cellulases by fusion with cellulose-binding domain of noncellulosomal cellulase engd from Clostridium cellulovorans. Proteins Structure, Function, and Bioinformatics, 2003, 50(4), 620 - 8. 

Cellulase complexes similar to the cellulosomes of C. thermocellum were also identified in other cellulolytic bacteria such as the mesophilic species C. cellulovorans, C. cellulolyticum, C. papyrosolvens, Bacteroides cellulosolvens, Acetivibrio cellulolyticus,Ruminococcus albus, and Ruminococcus flavefaciens and the thermophilic species Clostridium clariflavum and Clostridium josui. They... 

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