Cleavage protocols, peptide-resin mixture

The procedure for coupling 3a to the peptidyl-resin is similar to that for probe 2. After acid cleavage, a solution of the crude mixture is applied directly to an avidin-based affinity column and elution with buffers removes all underivatized chains. The peptide-containing column can be treated with an organic base to release free peptide (Protocol 5) or used directly for binding studies or affinity purification. 

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