Cleavage activity

T. van Vliet, F. van Schaik, W. H. Schreurs, and H. van den Berg, In vitro measurement of beta-carotene cleavage activity Methodological considerations and the effect of other carotenoids on beta-carotene cleavage, Int. J. Vitam. Nutr. Res. 66 (1996) 77-85. 

Note Tor, torulene LSO, hgnostilbene ISO, isoeugenol , full-length carotenoid cleavage , activity not known. 

Marasco, E. K., K.-l. Vay et al. (2006). Identification of carotenoid cleavage dioxygenases from Nosloc sp. PCC 7120 with different cleavage activities. J. Biol. Chem. 281(41) 31583-31593. 

Parvin, S. G. and B. Sivakumar. 2000. Nutritional status affects intestinal carotene cleavage activity and carotene conversion to vitamin A in rats. J Nutr 130(3) 573-577. 

Nakajima T, Ota N, Kodama T, Emi M. Isolation and radiation hybrid mapping of a highly polymorphic CA repeat sequence at the SREBP cleavage-activating protein (SCAP) locus. J Hum Genet 1999 44 350-351. 

Iwaki K, Nakajima T, Ota N, Emi M. A common Ile796Val polymorphism of the human SREBP cleavage-activating pro-... 

Chong, S., Montello, G.E., Zhang, A., Cantor, E.J., Liao, W., Xu, M.-Q., and Benner, J. (1998) Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucl. Acids Res. 26, 5109-5115. 

While enediyne natural products are efficient DNA cleaving agents, synthetic enediynes have been utilized widely to study the mechanistic details and obtain more efficient DNA cleavage activity. Simple enediynes (Fig. 8), with no binding units, show DNA cleavage albeit at very high concentra-... 

Spectra to be analyzed via PMF are derived from a protein sample been treated with an enzyme (e.g., trypsin) or other chemical (cyanogen bromide) with specific cleavage activity. The experimental m/z values for each peptide are converted into peptide masses and compared with the theoretical mass... 

Steinke M, Daniel C, Kirst GO (1996) DMSP lyase in marine macro- and microalgae intraspe-cific differences in cleavage activity. In Kiene RP, Visscher P, Keller M, Kirst GO (eds) Biological and environmental chemistry of DMSP and related sulfonium compounds. Plenum, New York, pp 317-323... 

For the 13 hammerhead FIFI16 mutants prepared by the reference 41 researchers, cleavage activity was significantly reduced in all cases. In four cases, at positions C3, A9, Gio.i, and A13, addition of the correct base (U, A, G, or C) rescued the activity of the ribozyme. A9X, for instance, is a ribozyme where the adenine base at position 9 has been removed to generate an abasic nucleotide (X = a nucleotide in which H substitutes for the removed base—see Figure 6.14). This altered ribozyme exhibited a 4 x 10 times lower catalytic rate than that for the wild-type ribozyme (krei = = 0.0004). When... 

After five cycles of selection and ampHfication, a population of single-stranded DNAs was enriched that catalyzed the Pb +-dependent cleavage at the ribose residue. This intramolecular cleavage activity was transformed into an inter-molecular reaction by separating the 38-nucleotide long catalytic domain from the 21-mer substrate which was cleaved specifically and with high turnover rates. Remarkably, the deoxyribozyme can perform well only with the special DNA/RNA chimeric oHgonucleotide substrate and cannot cleave a pure RNA substrate of the same sequence. 

Figure 14-8. Overview of pathways that regulate programmed cell death. Apoptosis may occur in response to signaling through either the extrinsic pathway or the intrinsic pathway. In each case, proteolytic cleavage activates an initiator caspase, caspase 8 or 9, either of which can cleave an effector caspase such as caspase 3. Apaf-1 is part of a large complex called the apoptosome that mediates the intrinsic pathway. Binding of an extracellular death ligand to its cell-surface receptor activates the extrinsic pathway.

The prokaryotic SuSy2 isoform purified from Anabaena sp. strain PCC 7119 [17] and the recombinant enzyme expressed in E. coli [18] revealed unfavorable kinetics and characteristics compared to those of the higher plant enzymes, e.g., a low affinity for sucrose, donor-dependent affinities for D-fructose, and complete loss of cleavage activity in the presence of Mn. ... 

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