Classification electrophoresis

Alaiya AA et al. Cancer proteomics from identification of novel markers to creation of artificial learning models for tumor classification. Electrophoresis 2000 21 1210-1217. 

Ferguson, KA, Starch-Gel Electrophoresis— Application to the Classification of Pituitary Proteins and Polypeptides, Metabohsm 13, 985, 1964. 

AC ADME ANS AUC BA/BE BBB BBM BBLM BCS BLM BSA CE CHO CMC CPC CPZ CTAB CV DA DOPC DPPC DPPH aminocoumarin absorption, distribution, metabolism, excretion anilinonaphthalenesulfonic acid area under the curve bioavailability-bioequivalence blood-brain barrier brush-border membrane brush-border lipid membrane biopharmaceutics classification system black lipid membrane bovine serum albumin capillary electrophoresis caroboxaldehyde critical micelle concentration centrifugal partition chromatography chlorpromazine cetyltrimethylammonium bromide cyclic votammetry dodecylcarboxylic acid dioleylphosphatidylcholine dipalmitoylphosphatidylcholine diphenylpicrylhydrazyl... 

F5. Farrer, M., Game, F. L., Adams, P. C., Laker, M. F., and Alberti, K. G. M. M., A simple sensitive technique for classification of apolipoprotein(a) isoforms by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Clin. Chim. Acta 207, 215-225 (1992). 

Ferguson, K. A. (1964). Starch-gel electrophoresis—Application to the classification of pituitary proteins and polypeptides. Metabolism 13(Suppl.), 985—1002. 

The most common classification scheme in electrophoresis focuses on the nature of electrolyte system. Using this scheme, electrophoretic modes are classified as continuous or discontinuous systems. Within these groupings the methods may be further divided on the basis of constancy of the electrolyte if the composition of the background electrolyte is constant as in capillary zone electrophoresis, the result is a kinetic process. If the composition of the electrolyte is not constant, as in isoelectric focusing, the result is a steady-state process. 

In electrophoresis there are two main contributions to the movement of analyte ions first, the electrophoretic mobility of the analyte ion itself and, second, the speed and direction of the electroosmotic flow (EOF). The EOF may be induced to travel in the same direction as the analyte ions or in the opposite direction from the analyte ions, or it can be suppressed so that the flow is negligible. Figure 4.2 shows the classification of electrophoresis according to the contribution of the electroosmotic flow. 

Figure 4.2 Classification of electrophoresis according to the contribution of the electroosmotic flow. CZE, Capillary zone electrophoresis MECC, micellar electrokinetic capillary chromatography CEC, capillary electrochromatography cIEF capillary isoelectric focusing cGE, capillary gel electrophoresis.

Capillary electrophoresis methods may be classified according to the nature of the electrolyte system or according to the contribution of the electroosmotic flow. The former classification is the most popular, and two types of systems have been identified ... 

Significant improvements in the technologies of high-resolution two-dimensional Polyacrylamide Gel Electrophoresis (2-D PAGE) and Mass Spectrometry (MS) have marked the start of proteome analysis. Proteomics permits the analysis of thousand of proteins simultaneously, and have the potential to identify markers for early detection, classification and prognosis of diseases, as well as pinpointing targets for improved treatment outcomes [42]. 

We should note that almost every system, even those in the c class, contains a discontinuity in pi located at the walls, electrodes, and other nonpermeable barriers that define the physical limits of the system. However, although walls represent an abrupt pi barrier, we do not automatically put separation in the d class because of ttfelm. If they are passive container walls simply holding the system in place, or if they are electrodes which charged particles never touch (as in electrophoresis), a d classification is not justified. However, if the walls play a major part in structuring the separation (such as membrane filters or the outer wall of a centrifuge cell which structures the solute concentration profiles in equilibrium sedimentation), those walls convey a d classification to the method. 

Classification of Organic Solvents in Capillary Electrophoresis Ernst Kenndler... 

In capillary electrophoresis (CE), several criteria can be applied to classify solvents [e.g., for practical purposes based on the solution ability for analytes, on ultraviolet (UV) absorbance (for suitability to the UV detector), toxicity, etc.]. Another parameter could be the viscosity of the solvent, a property that influences the mobilities of analytes and that of the electro-osmotic flow (EOF) and restricts handling of the background electrolyte (BGE). For more fundamental reasons, the dielectric constant (the relative permittivity) is a well-recognized parameter for classification. It was initially considered to interpret the change of ionization constants of acids and bases according to Born s approach. This approach has lost importance in this respect because it is based on too simple assumptions limited to electrostatic interactions. Indeed, a more appropriate concept reflects solvation effects, the ability for H-bonding, or the acido-base property of the solvent. 

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