Cibacron F dyes

Fluorination and iodination reactions are used relatively littie in dye synthesis. Fluorinated species include the trifluoromethyl group, which can be obtained from the trichi oromethyl group by the action of hydrogen fluoride or antimony pentafluoride, and various fluorotria2iQyl and pyrimidyl reactive systems for reactive dyes, eg, Cibacron F dyes. 

Cibacron C dyes Cibacron E Cibacron E dyes Cibacron F... 

RP-HPLC was also employed for the investigation of the hydrolysis behaviour of two fluorotriazine reactive dyes Cibacron blue F-R and Cibacron yellow F-4G. Chromatographic measurements were performed in an ODS column (50 X 4.6 mm i.d. particle size 5 /jm) at room temperature. The mobile phase was ACN-0.05 M ammonium acetate buffer containing 1 mM acetyhrimethylammonium bromide (47 53, v/v). Flow rates were 0.8 and 0.6 ml/min depending on the dyes to be separated. Dyes were detected at 275 nm. The hydrolysis of dyes was investigated both in the absence and presence of... 

Fig. 3.124. Chromatograms of a 1 1 dye mixture (a) 40 min before addition of Na2C03, (b) 10 min after addition of Na2C03, and (c) 60 min after addition of Na2C03 flow rate = 0.6 ml/min, other conditions are described in the text. The peak at 4.57 min in (a) and the same peak (smaller in size) at 4.54 min in (b) are both attributed to the functional group of cibacron yellow ( — F) the peak at 7.54 min in (a) and the same peak (smaller in size) at 7.40 min in (b) are both attributed to the hydrolysed part of the functional group of cibacron yellow the peaks at 5.07 min in (b) and 5.22 min in (c) are attributed to the hydrolysed part of the functional group of cibacron blue. Reprinted with permission from A. Zotou et al. [175].

HTMA adsorption is enhanced by counter ions on the aqueous side of the interface - F", CP, and Br , in this order [84]. Wiles et al. found similar potential-dependent adsorption of a triazine dye, a derivative of Cibacron Blue F3GA, at the 1,2-dichloroethane-water interface by electrocapillary and capacitance measurements [85]. A pronounced potential dependence of the adsorption of ionic dyes at the ITIES has recently been confirmed for the adsorption of 2-(n -octadecylamino)naph-thalene-6-sulfonate at the 1,2-dichloroethane-water interface by using the optical second harmonic generation technique (Fig. 7) [86]. 

Besides attempts to construct direct potentiometric immunosensors, other approaches are under investigation (piezoelectric, optical, etc.) and to date seem to be more acceptable[27]. An affinity electrode based on mutual interaction of a dye and protein has been constructed by Lowe (Fig. 15, [306]). He immobilized an anionic triazine dye (Cibacron Blue F 3G-A) to an oxidized metal electrode. This affinant specifically binds human serum albumin from plasma. The response of this albumin electrode was linear up to 15 g of human serum albumin per ml and faster than for the immunologically sensitized metal electrode [304]. 

Boyer and Hsu [1] studied the equilibrium adsorption of alcohol dehydrogenase onto Cibacron Blue F-3GA affixed to Sepharose CL-6B in a potassium phosphate, pH 7.0 mobile phase at 4°C and found that depending on the concentration of the immobilized dye, Ka fell in the range of 1.0 - 2.6T0- M. The relative effect of ligand concentration on Ka for various proteins based on the results of Boyer and Hsu are shown in Figure 4. Arnold and Blanch [7] have found that the Ka values fall within the range 1.1 - 1.3 10- M for bovine serum albumin adsorbing to Cibacron Blue Sepharose CL-4B. They also reported the variation of Ka for... 

Moreover, the triazine dyes (e.g.. Cibacron blue F 3 G-A and Procion red HE-3 B) [ 188], [ 189] mentioned above are also used as ligands for the purification of these proteins. Triazine dyes can replace ligands of the NAD group because they have similar spatial structures. In addition, they bind a series of blood proteins (e.g., albumins, lipoproteins, clotting factors, and interferon). 

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