Chromatography Using Molecularly Imprinted Polymers

Preparative separations are also possible. For example, 30 mg of the racemate of 7b were effectively resolved by 20 g of polymer[143]. 

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Khasawneh MA, Vallano PT, Remcho VT. Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorhents. II. Covalent imprinted polymers. J Chromatogr A 2001 922 87-97. 

Zhu, XL Yang, J Su, QD et al. Seleetive Solid-Phase Extraction Using Molecularly Imprinted Polymer for the Analysis of Polar Ogmaophosphorus Pesticides in Water and Soil Samples. Journal of Chromatography A, 2005,1092,161-169. 

Marcel Dekker, Inc. All rights reserved. This material may not be used or reproduced in any form without the express written permission of Marcel Dekker, Inc. 2 Molecularly Imprinted Polymers for Affinity Chromatography... 

Javanbakht, M., Shaabani, N., Akbari-adergani, B. (2009). Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography, I Chromatoar. B. 877, 2537-2544. 

In addition to studies where the nature of the recognition events per se has been the major issue, a number of application areas have been explored for imprinted matrices viz. (A) Chromatography, where the imprinted polymer is used as the stationary phase for separation and isolation (Chapter 20). This application is based on the fact that the imprinted polymer has a better retention for the template molecules than others. A related area of application is solid-phase extraction, where the imprinted polymer is used as a sponge to concentrate the molecule of interest (Chapter 23). (B) Immunoassay-type analyses in which molecularly imprinted polymers are used as antibody and receptor mimics (Chapter 25). (C) Catalysis where the molecularly imprinted polymers are used as enzyme mimics (Chapter 24). (D) Sensors and biosensor-like devices where the molecularly imprinted polymer is the recognition element (Chapters 26 and 27). 

A molecularly imprinted column for liquid chromatography can be used not only to separate analytes, but also to selectively extract analytes from complex samples. This technique is called on-line Molecularly Imprinted Solid Phase Extraction (on-line MISPE), and it combines the high extraction efficiency of reverse phase SPE for aqueous samples with the high selectivity of the molecular-imprinted polymers. Examples of successful selective extraction and clean-up are reported in Figs. 9 and 10. 

Table 3 Examples of Molecular Imprinted Polymers Used as Stationary Phases in Chiral Chromatography...

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