Chromatography, organic synthesis

Thin-Layer Chromatography TLC) The function of TLC in organic synthesis is primarily one of allowing the experimenter to follow the progress of the reaction without actually interrupting the reaction. Since successful TLC can be carried out on a minute scale, only a very small fraction of the reaction mixture need be withdrawn and subjected to analysis. The following example of the TLC analysis of the chromic acid oxidation of borneol, described by Davis (3), is a useful model. 

The purpose of preparative-scale liquid chromatography is the isolation of materials conforming to a specified purity in cUBOunts that depend on the intended use of the product [8,570-572]. Possible uses Include the isolation of materials for structural elucidation, for biological or sensory evaluation, for organic synthesis or commercial applications. The scale of the... 

Zhang and Nunes [92] studied the structure and the generation mechanism of a novel degradation product formed by oxidatively induced coupling of miconazole nitrate with butylated hydroxy toluene in a topical ointment studied by high performance liquid chromatography-electrospray ionization mass spectrometry and organic synthesis. 

Notwithstanding the reduced reaction times and improved yields, the need to use column chromatography to purify the target 1,4-DHP encumbers the application of the procedure for the fast preparation of screening libraries. To address the purification issue, various phase-separation techniques could be employed, such as solid-phase organic synthesis (SPOS). 

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