Chromatography liquid, general principle

By analogy to the above technique, gas chromatography is considered a useful tool to obtain data for gas and vapor adsorption on polymeric surfaces. In contrast to liquid chromatography, the general principle of the IGC technique is well established for the characterization of polymeric materials this technique called inverse gas chromatography (IGC), enables the study of various polymeric properties, including interfacial properties (15-18). 

Optical Spectroscopy General principles and overview, 246, 13 absorption and circular dichroism spectroscopy of nucleic acid duplexes and triplexes, 246, 19 circular dichroism, 246, 34 bioinorganic spectroscopy, 246, 71 magnetic circular dichroism, 246, 110 low-temperature spectroscopy, 246, 131 rapid-scanning ultraviolet/visible spectroscopy applied in stopped-flow studies, 246, 168 transient absorption spectroscopy in the study of processes and dynamics in biology, 246, 201 hole burning spectroscopy and physics of proteins, 246, 226 ultraviolet/visible spectroelectrochemistry of redox proteins, 246, 701 diode array detection in liquid chromatography, 246, 749. 

In this chapter, high-performance liquid chromatography of oligomers and (high) polymers (polymer HPLC) will be briefly presented. As mentioned in Section 16.1, there exist several monographs, chapter in books, and review papers on this subject, for example [1-33], Most of them contain numerous examples of the HPLC separation and molecular characterization of particular macromolecular substances. Therefore, this chapter discusses almost exclusively the general principles of polymer HPLC and only few selected examples of practical applications will be mentioned for illustration. 

W. M. A. Niessen and A. P. Tinke, Liquid chromatography-mass spectrometry. General principles and instrumentation , 7. Chromatogr. 703 37-57 (1995). 

Niessen, W.M.A. and Tinke, A.P. (1995) Liquid Chromatography-Mass Spectrometry. General principles and instrumentation,/. Chromatogr. A, 703(1-2), 37-57. 

As far as liquid phase chromatography on columns is concerned it is probably true to say that the division into adsorption and partition methods is of practical, rather than theoretical, significance. The importance of adsorption varies from system to system and is mentioned briefly in connection with the different supports described below. The moving phase in partition chromatography may be a liquid or a gas, and the general principles are the same in each case. 

Xu FG, Zou L, Liu Y, Zhang ZJ, Ong CN. Enhancement of the capabilities of liquid chromatography—mass spectrometry with derivatization general principles and applications. Mass Spectrom Rev 2011 30 1143-72. 

Nikolova-Damyanova, B. (1997). Reversed-phase high-performance liquid chromatography general principles and applications to the analysis of fatty acids and triacylglycerols. In Advances in Lipid Methodology - Four, W.W. Christie (ed.). Oily Press. 

It is not our aim to produce another general book on liquid chromatography. The chapter on background is therefore rather brief and it touches on many areas which are not necessarily directly related to separation techniques but which are relevant to derivatization. This chapter should enable the practical worker to recollect some of the fundamental principles involved. The third chapter is concerned with instrumentation. 

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