Chromatography a key method for separation and identification of biomolecules

Chromatography is the most widely used separation technique in hioanalytical chemistry. There are several different modes of chromatography that are based on different physical and chemical attributes of biomolecules, which include separation based on ionic charge, solute partitioning, molecular size, and adsorption properties. Subsequent sections provide more information on the different modes of chromatography and their application. [Pg.142]

There are two major classifications of chromatography according to whether the separation takes place on a planar surface (i.e. flat sheet, e.g. paper or thin-layer [Pg.142]

Chromatography method by classification Stationary phase Mobile phase Separation mechanism [Pg.142]

Ion-exchange Resin/matrix Liquid Ionic charge [Pg.142]

Size-exclusion Porous resin Liquid Size of molecule [Pg.142]

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