Primary structure chromatographic separation

MDPF is closely related in structure to fluorescamine. Both primary and secondary amines react with MDPF in acetonitrile, but only primary amines form fluorescent pyrrol inones. Secondary amines form non-fluorescent aminodienones. By reacting with ethanolamine, the latter produce fluorescent products. Their stability is adequate for chromatographic separation [258, 259]. 

In this section a variety of analytical separations reported in the literature are reviewed to show the wide structural diversity of eluite which can be separated by RPC and to assist the reader in becoming similar with the use of this fluid chromatographic technique. The descriptions are ar-ranged according to the matrix in which an analyte is found or the area of - h istry in which the samples are generally encountered. Thus theophylline, for example, is regarded as a nucleotide and, for the most part, its analysis in food samples is found with appropriate cross references. On the other hand, the separations of pharmaceuticals found in serum, urine, and pharmaceutical samples are cited separately. It is hoped that this method of classification may serve the purposes of those wh e analytical interests are incidental to their primary research pursuits. 

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