Chromatographic Separation of Amino Acid Mixtures

Plate 4. Stanford Moore, 1. (1913-1982) and William H. Stein (1911-1980). In the Rockefeller Institute, New York City 

An even more sensitive reagent for this purpose was introduced by S. Udenfriend and collaborators in 1972, M. Weigele s fluorescamine (III in Fig. 4) which at room temperature with primary amines quickly forms strongly fluorescent pyrrolinones (IV) fluorometrically measurable even in the picomole range [14]. 

On the way to the final concept Moore and Stein also made use of results which had been obtained in other laboratories. In 1941 A.J.P. Martin (Plate 31) and R.L.M. Synge (Plate 44) in England introduced partition chromatography 

Whereas partition chromatography in this field was soon superseded by an ion exchange method (see above), it achieved great success with cellulose as a support, as paper chromatography. According to Consden, Gordon and Martin 

Filter paper, in a simple self-made device, as a support for electrolytes was recommended for the separation of amino acids as ionized substances due to their 

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