Chromatographic retention behavior fluids

The retention behavior of the eluite alone cannot be used for the unambiguous identification of the compound under study. This is especially important in the analysis of physiological fluids where several different compounds in the matrix may have similar retention characteristics. Thus, a comparison of retention times for the eluting compounds with those of reference compounds that have been previously chromatographed under identical conditions, can only be used for tentative identification. For this identification procedure, knowledge of the type of compounds and their relative levels in the sample is usually necessary. 

Trimethylsilyl derivatives of ten hydroxy- and methoxyhydroxyflavonoids have been studied by the GC-FTIR technique." " The correlation found between retention and gas-phase IR data was used in structural identification of compounds having very similar chromatographic behavior. The shift of the carbonyl frequency gave information on the presence of substitution. Some hydroxy- and methoxy-substituted flavones have been studied following carbon dioxide supercritical fluid chromatography on polymethylsiloxane capillary columns using flame ionization and FTIR detection." " " ... 

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