Chromatographic definition

Descending Paper Strip. First, individual fractions spaced five fractions apart were chromatographed. Definite GA3 response was observed in fraction 10 or the spike, weaker response in fraotion 15, and no response in the natural extract single fractions. To avoid the possibility of dissipating the fractions in individual runs below the detection limit, the remaining fractions were combined in groups... 

Substituting into the standard chromatographic definition of capacity factor (Equation 17), one obtains Equation 18. 

In this book, we use alternately the chemical engineering and the chromatographic definitions of the internal porosity, the mobile phase and the equilibrium constant, depending on the choice made by the original authors whose work is discussed. We try to indicate clearly which definition has been used in each case. 

Table 3.2 contains a summary of the most important chromatographic definitions and equations, and a complete list of symbols and acronyms is included in Appendix I. 

Let us now consider some chromatographic definitions, which are known from the theory - which, by the way, was developed originally for the GC and much later for isocratic LC separations. The derivation of the formulas used below is omitted, they are only used to elaborate the consequences for practical optimization. For a more detailed discussion, see Refs. [2-4] and in particular [Ij. 

To understand these effects, several important chromatographic definitions are discussed in this section. Figure 1 shows a typical chromatogram with the most important parameters characterizing a chromatographic separation. 

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