Chromatium purification

The visible absorption spectrum of a partially purified enzyme of Chromatium vinosum indicates a high-spin cytochrome c (36.49). Whether cytochrome c itself can be regarded as the real enzyme or whether c is only attached to the enzyme during the purification procedure, still remains unclear. On the other hand, Ulbricht (35) could clearty demonstrate that the enzymes of Chromatium gracile and Chromatium minutissimum have no cytochrome character. 

NADH dehydrogenase and, 189 ubiquinone reductase and, 178,182,183 Cholesterol, side chain cleavage, 83, 84-85 Choline, oxidation to betaine, 260 Choline dehydrogenase electron transport system and, 261-263 properties, 260-201 Chromatium sulfate reduction by, 281 transhydrogenase of, 54 function of, 80 molecular properties, 58, 69 purification, 55, 56 Chromium... 

Fig. 4. Top Spectrum of the 150-//S decay phase induced in pre-reduced, pre-iiiuminated PS-i (D144) particles by 20-ns, 600-nm iaser flashes (data points drawn as smail circles and connected by solid line) the dotted difference spectrum represents changes due to P700 photooxidation, AA[P700 -P700J, taken from reference (16) bottom difference spectrum due to reduction of vitamin K, to its semiquinone anion in methanol. Figure source Brettel, Sdtif and Mathis (1986) Flash-induced absorption changes in photosystem I at low temperature evidence that the electron acceptor is vitamin K,. FEES Lett 203 223 the original in vitro spectrum for the reduction of vitamin K, to the semiquinone was from EJ Land, replotted in Romijn and Amesz (1977) Purification and photochemical properties of reaction centers of Chromatium vinosum. Evidence for the photoreduction of a naphthaquinone. Biochim Biophys Acta...

LiebeigeseU, M., Sonomoto, K., Madkour, M., Mayer, F., Steinbuchel, A. Purification and characterization of the polyfhydroxy alkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules. EurJBiochem 1994,226,71-80. 

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