Chondrogenic gene expression

Barry, E et aL, Chondrogenic differentiation of mesenchymal stem cells from bone marrow Differentiation-dependent gene expression of matrix components. Exp Cell Res, 2001. 268(2) 189-200. 

Goat bone marrow stromal cells Perfusion 0.1 ml/ min, static Starch-PCL fiber mesh ITS. FBS. dexamethasone. 10 ng/mL pi 28 days Bioreactor groups significantly higher protein and gene expression for chondrogenic markers compared to static controls... 

Zhao, G.Q., Eberspaecher, H., Seldin, M.F., de Crombrugghe, B. 1994. The gene for the homeodomain-containing protein Cart-1 is expressed in cells that have a chondrogenic potential during embryonic development. Mech. Dev. 48, 245-254. 

Human MSCs isolated from two different tissues, adipose tissue and placenta, automatically formed 3D spheroids when cultured on chitosan and chitosan-hyaluronan (chitosan-HA) membranes. Cells in these spheroids remained relatively undifferentiated, because sternness marker genes were well maintained, and blocking spheroid formation decreased the expression of these marker genes. Moreover, the formation of 3D cell spheroids may also have higher chondrogenic differentiation capacity upon TGF-p3 induction. 

Equine Monolayer, fibrin disks 0.5 ng/mL TGF-Pl monolayer 6 days. 0. 100 ng/mL IGF-1 fibrin disks 13 days Monolayer with TGF-Pl increased proliferation, disks with both growth factor treatments increased gene and protein expression for chondrogenic markers over controls... 

Human Monolayer or pellet. 5% Oj or 20% Oj ITS. dexamethasone, 10 ng/mL TGF-(il 28 days Histology, gene and protein expression all chondrogenic markers upregulated in 5% Oj... 

Human Alginate ITS, dexamethasone, 10 ng/mL TGF-Pl, 10 ng/mL TGF-P3, 100 ng/mL IGF-1, 500 ng/mL BMP 6 7 days Gene and protein expression indicate BMP 6 alone significantly more efficient at upregulating chondrogenic markers than all other growth factors/combinations... 

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