Cholinesterase inhibition Warburg

A2. Adie, P. A., and Tuba, J., A new type Warburg vessel and its application to the study of some kinetics of cholinesterase inhibition. Biochim. Biophys. Acta 50, 70-75 (1961). A3. Agarwal, D. P., Schwenkenbecher, S., Srivastava, L. M., and Goedde, H. W., Spektrophotometrische Bestimmungsmethode fiir Serumcholinesterase (EC 3.1.1.8)-varianten mit Succinylbischolin als Substrat. Z. Klin. Chem. Klin. Biochem. 13, 133-135 (1975). 

Allen, 27 April) 730 (Slater 4 May) 743 (Verallo, 6 May) 745 (Maddison, 6 May). Michel and Warburg were the then existing methods of measuring cholinesterase inhibition levels. 

MI, Folder 2B, Exhibit MNJ/30, FTP 399, Percutaneous Toxicity of G-Compounds , ii January 1954, pp. 267-318, here p. 272. MI,Transcript, Day 60, p. 65 lists Sammons ChE level as 95 and 92 respectively, depending on whether the Warburg or Michel method of measurement was used. Whereas the Coroner used the data recorded in the chamber books, PTP 399 used the mean cholinesterase inhibition level. 

Berberine inhibits oxidative decarboxylation of yeast pyruvic acid (310) the same dose has, however, no effect upon aerobic glycolysis, Warburg s respiratory enzymes, indophenol oxidase, etc. Berberine and tetrahydroberberine have an inhibitory effect on oxidation of (+ )-alanine in rat kidney homogenates (498). Berberine and palmatine show a specific inhibitory effect upon cholinesterase in rabbit spleen and on pseudocholinesterase in horse serum (499). Berberine inhibits cellular respiration in ascitic tumors and even in tissue cultures (500-502). The specific toxic effect of berberine on the respiration of cells of ascitic tumors in mice was described (310). The glycolysis was not found to be affected, but the uptake of oxygen was smaller. Fluorescence was used in order to demonstrate berberine in cellular granules. Hirsch (503) assumed that respiration is inhibited by the effect of berberine on the yellow respiratory enzymes. Since the tumorous tissue contains a smaller number of yellow respiratory enzymes than normal tissue it is more readily affected by berberine. Subcutaneous injections of berberine, palmatine, or tetrahydropalmatine significantly reduce the content of ascorbic acid in the suprarenals, which is not affected by hypophysectomy (504). 

Neely et al. (1964) have prepared a series of aryl N-methyl methylphosphorami-dates and have examined them for inhibition of fly-head Musca domestica L.) cholinesterase by a standard Warburg manometric method (Metcalf, 1949). The type formula for N-methyl methylphosphoramidates is given by LXXXVIII. 

---