Choline phosphorylcholine formation with

It is also known from the work of Sribney and Kennedy (1958) that many tissues contain an enzyme (CDP-choline ceramide cholinephosphotransferase EC 2.7.8.a) capable of transfering phosphorylcholine from CDP-choline to ceramide with the formation of sphingomyelin ... 

CDP-choline has been characterized and synthesized (Kennedy 1956). It occurs in a number of tissues (Kennedy and Weiss 1956) including brain (Ansell and Bayliss 1961) and yeast (Bebgeb and Gimenez 1956). The properties of the enzyme phosphorylcholine-cytidyl transferase (CTP cholinephosphate cytidylyl-transferase EC 2.7.7.15) catalysing Reaction 11 have been studied by Bobken-HAGEN and Kennedy (1957), who showed that the enzyme readily catalyses the reverse reaction, i.e. the pyrophosphorolysis of CDP-choline, with the formation of phosphorylcholine and CTP. 

The enzyme catalysing this reaction, phosphorylcholine-glyceride transferase (CDP-choline 1,2-diglyceride cholinephosphotransferase EC 2.7.S.2), was partially purified from liver by Weiss, Smith and Kennedy (1958), who showed that it catalyses the reverse reaction, i.e. the cytidylolysis of lecithin with the formation of CDP-choline and D-a,j8-diglyceride. 

Thus CDP-choline is the precursor of three phosphatides (Figure 3). It may donate phosphorylcholine to D-a,/5-diglyceride with the formation of lecithin... 

---