Cholera toxin interaction between ganglioside

Ellipsometry can follow the interactions between two types of biological macromolecules, the first of those two bound physically to the surface, the other acting from the solution. The binding of conconavalin A to adsorbed mannan 180) and of cholera toxin to adsorbed ganglioside t83) are examples. The adsorption of complement factors to an antibody-coated surface was monitored by ellipsometry and a modification of the same method was used for quantification of migration inhibition of human polymorphonuclear leucocytes 182). Interaction of proteins and cells with affinity ligands covalently coupled to silicon surfaces has been also studied 183). 

Picking WL, Moon H, Wu H, et al. (1995) Fluorescence analysis of the interaction between ganglioside GMi-containing phospholipid vesicles and the B subunit of cholera toxin. In Biochim. Biophys. Acta 1247 65—73. 

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