Chloride test for

Acetyl chloride test (for primary and secondary amines). In a semi-micro test-tube (75 x 10 mm.) treat 0-5 ml. (or 0-5 g.) of the compound with acetyl chloride drop by drop. Note whether reaction occurs. If no solid separates, pour the contents of the tube into 3 ml. of water and neutralise the aqueous layer with solid sodium bicarbonate. Observe whether a product different from the original compound is produced. 

Micro distillation is sometimes required, e.g. in the chromyl chloride test for a chloride (see Section IV.14). The apparatus depicted in Fig. 11.59 is suitable for the distillation of very small quantities of a mixture. A micro crucible or a micro centrifuge tube may be employed as a receiver. 

Data Sheet 13.2 Ferric chloride test for phenolics Results for some deciduous and coniferous trees... 

Negatively substituted phenols such as nitrophenols, aldehydophenols, and polyhalophenols are sufficiently acidic to dissolve in 5% sodium bicarbonate. See Group I(i), page 579, for the iron(III) chloride test for phenols however, this test is not completely reliable for these acidic phenols. 

M20. Meulemans, O., The ferric chloride test for phenylpyruvic add in urine. Clin. Chim. Acta 6, 152 (1960). 

Chemical Tests. Chemical classification tests (see Chapter 9) are also of value to establish the identity of these compounds. Perform the ferric chloride test for phenols. Is a positive result obtained for each compound Does the sodium fusion test detect the presence of nitrogen The test for nitro groups might also be performed. The phenyl- or a-naphthylurethane derivatives of the phenols may be prepared to further establish their identity (see Chapter 9, Preparation of Derivatives). 

Fatty acid biosynthesis, 159 Ferric chloride, test for hydrazones, 211 Flavin enzymes, 33,160 Fluorescein, 563, 664, 667 preparation of, 665... 

Soloway, S. and Wilen, S.H. 1952. Improved Ferric Chloride Test for Phenols. Analytical Chemistry, 24, (6), 979-983. 

The acetyl derivatives are most often used for the preparation of derivatives of primary and secondary amines. Often they may be isolated in connection tvith the acetyl chloride test for amines (page 144) but usually it is best to prepare them from acetic anhydride. The reaction mixture is poured into water, warmed to decompose the excess of anhydride, cooled, and filtered. The product may be crystallized from water or dilute alcohol. 

Cinnamon Bark Oil. Use 1 g for the determination of aldehydes. By experiment, the usually accepted ferric chloride test for absence of leaf oil was found to admit from 30 to 40 per cent adulteration. A determination of phenols should be made, not more than 10 per cent being present in bark oils. 

B. Modified Ferric Chloride Test, for Enolic Compounds... 

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