Chitin homogeneous solution reactions

Homogeneous Solution Reactions of Cellulose, Chitin, and Other Polysaccharides... 

McCormick, C.L. and D.K. Lichatowich, "Homogeneous Solution Reactions of Cellulose, Chitin, and Other Polysaccharides to Produce Controlled Activity Pesticide Systems," submitted... 

McCormick, C.L. and Lichatowhich, D.K. (1979) Homogeneous solution reactions of cellulose, chitin and other polysaccharides to produce controlled... 

In another oudine, cellulose was complexed with cuprammonium ions (Nicoll and Conaway, 1943). Lately, laboratory-scale isolation has relied on polar aprotic solvents and solvent systems, e.g., dimethylsulfoxide, pyridine, Af,7V-dimethylacetamide-lithium chloride, and l-methyl-2-pyrrolidinone-lithium chloride (Baker et al., 1978 McCormick and Shen, 1982 Seymour et al., 1982 Arnold et al., 1994). These solvents have enabled such homogeneous17 reactions as O- and N-derivatization of cellulose and chitin (Williamson and McCormick, 1994) and selective site chlorination (Ball et al., 1994). Dimethylsulfoxide was the solvent in a homogeneous reaction of cellulose and paraformaldehyde, prior to isolation of purified cellulose (Johnson et al., 1975). In yet another outline, paraformaldehyde enabled superior quality extracts when the parent tissues were presoaked in this solution (Fasihuddin et al., 1988). 

Chitinase from Bacillus sp. was added to a solution of Xyl (l— 4)GlcNAc oxazoline 13 dissolved into a phosphate buffer. The consumption rate of 13 was accelerated by the enzyme addition and chitin-xylan hybrid polysaccharide 14 was produced. During the polymerization, the reaction proceeded homogenously throughout the reaction. The yield was 76% and its average molecular weight value of 14 was M = 1500 with MJMa = 1.76, determined by GPC. Furthermore, MALDI-TOF mass spectrum of 14 indicated the peaks at every m/z 335, which corresponds to the molecular mass of the repeating disaccharide unit. 

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