Hybrid polysaccharides chitin-chitosan

The target glycosidic linkages for the synthesis of a chitin-chitosan hybrid polysaccharide are (1—>4)-j8-D-glucosaminide and (1—>4)- 8-iV-acetyl-D-... 

After the complete consumption of 7, the reaction mixture was analyzed by MALDI-TOF/MS, H and C NMR measurements. The MALDI-TOF/MS spectrum showed peaks at every mtz 364, which is corresponding to the molecular mass of the repeating disaccharide unit of 8. H and C NMR spectra also supported that the resulting product is a chitin-chitosan hybrid polysaccharide 8. 

The yields and Mn value of the resulting chitin-chitosan hybrid polysaccharides 8 under various reaction conditions were summarized in Table 2. The yields of 8 were around 60-75% and there were little significant differences in catalysis between these two enzymes except for the reaction time. However, A4 value of 8 reached 1600 and 2020 with using chitinases from Bacillus sp. and Serratia marcescens, respectively, which correspond to 8-10 (n = 4-5) and 10-12 (n = 5-6) saccharide units, respectively. These results Indicated that values of polysaccharides are controllable to some extent by selecting enzymes from different origins. 

Makino, A., K. Kurosaki, M. Ohmae, and S. Kobayashi. 2006b. Chitinase-catalyzed synthesis of altematingly V-deacetylated chitin A chitin-chitosan hybrid polysaccharide. Biomacromolecules 7 950-957. 

A wide range of polymers from solution, sol-gel suspension, or melt can be electrospun into nanofibres. To date, over 200 types of various materials including natural polymers, synthetic polymers and hybrid blends have been used to obtain electrospun fibres [36]. Due to the practicality, mouldability, flexibility, lightness, durability and chemical and physicochemical stability, natural polymers are more preferable to synthetic polymers. Therefore, four major classes of biopolymers including proteins, polysaccharides, deoxyribonucleic acids (DNAs) and lipids as well as their derivatives have been fabricated into electrospun scaffolds [37, 38], The most popular natural polymers include chitosan, collagen, gelatin, casein, hyaluronic acids, silk protein, chitin and fibrinogen [37-43]. 

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