Chick embryo chorioallantoic membrane

Marzullo, A., Vacca, A., Roncali, L., Pollice, L. and Ribatti, D. (1998). Angiogenesis in hepatocellular carcinoma an experimental study in the chick embryo chorioallantoic membrane. Int. J. Oncol. 13, 17-21. 

Nguyen, M., Shing, Y. and FoUanan, J. (1994). Quantitation of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane. Microvasc Res. 7, 31-40. 

Ribatti, D., Urbinati, C., Nico, B., Rusnati, M., Roncali, L. and Presta, M. (1995). Endogenous basic fibroblast growth factor is implicated in the vascularization of the chick embryo chorioallantoic membrane. Dev. Biol. 170, 39-49. 

Aroylindoles bind to the colchicine site of p-tubulin, however, and in contrast to colchicine (1), vincristine (2a), nocodazole (13) or taxol (3), they showed no significant influence on the GTPase activity up to 10 pM [48,49]. In addition, angiogenesis in the chick embryo chorioallantoic membrane (CAM) assay was inhibited by D-64131 and analogue. Since 2-aroylindoles are easy to synthesise (Scheme 2), the role of the indole substitution pattern was studied in more detail. 

Tissue culture experiments have shown antiviral activity for tetra-methyldipicrylamlne (TMP) against myxovirus, picornaviruses and group A arboviruses O with antiviral activity also reported for 2-(pyridyl)-benzthlazole analogs against Influenza A/fr8 and parainfluenza 1 in chick embryo chorioallantoic membranes ... 

Embryo, chorioallantoic membrane injection on Chicks had a dose-dependent decrease in bone organic... 

A variety of other calcium transport systems are associated with Ca21-activated ATPases. The extraembryonic structure, the chorioallantoic membrane, of the chick embryo is responsible for the translocation of over 120 mg of eggshell calcium into (he embryo during development. The enzyme responsible for this is a (Ca2+, Mg2+)-ATPase with Km values for Ca2+ of 30 p,mol dm-3 and 0.3 mmol dm-3, and a molecular weight of 170 000. The enzyme can be crossiinked and co-isolated with a calcium-binding protein.158 Transport of Ca2+ is also associated with (Ca2+, Mg2+)-ATPases in neutrophil plasma membranes,159 transverse tubule membranes from rabbit skeletal muscle,160 rabbit myocardial membrane,161 endoplasmic reticulum,162 sar-colemma,163 brain microsomes,164 the Golgi apparatus165 and rat liver plasma membranes.166... 

Ex vivo models, such as mouse bladder (Poste et al., 1980) or human amnion (Russo et al., 1986) either denuded of epithelium and/or reseeded with endothelial cells (Foltz et al., 1982) may be considered too simplified systems. According to Kim et al. (1998), these recapitulate poorly the structure of the blood vessels and, in particular, small vessels. .. where most of the cancer cell invasion is believed to take place . These authors describe an interesting alternative where cells are inoculated on the chick chorioallantoic membrane (CAM) of an artificially created air sac in chick embryo. To detect and quantitate tumor cells actively penetrated in the ventral lower CAM , genomic DNA is extracted and used as a template for human Alu sequence identification by PCR. These sequences, unique to human and higher primate DNA, are repetitive... 

Barnhill, R. L. and Ryan, T. J. (1983). Biochemical modulation of angiogenesis in the chorioallantoic membrane of the chick embryo. J. Invest. Dermatol. 81,... 

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