Chemiluminescence emission intensity calibration

Effect of flow rate. The effect of flow rate on the chemiluminescence emission intensity was studied over the range 0.50-3.00 mL/min in each stream. An increase in the emission intensity was obtained with increase in flow rate in range 0.50-2.00 mL/min. Thus the chosen optimal flow rate was 2.00 mL/min in each channel. Analytical performance. Calibration graphs were obtained for each of the CL system described above in Fig. 1. Under the optimum conditions described above, hydroxylamine concentration was linear over the range 3.00x 10 7- 5.00x10 6 M... 

The reaction now affords investigators another valuable tool because the absolute intensity of the chemiluminescence has been carefully measured. It can thus be used as a standard light source against which other chemiluminescent reactions can be measured without the need of detector calibrations and geometry corrections. This convenience is due largely to the fine work of Fontijn, Meyer, and Schiff,145,147 who used chemical actinometry to measure the emission from a discharge-flow system. In their first report on this reaction, they determined the value of k12 as 1.0 x 104 M x sec-1 for emission in the... 

Luminol, whose chemiluminescence in the presence of hydrogen peroxide, can readily be catalysed by horseradish peroxidase (HRP), has found an application in the calibration tests for enzyme-linked immunosorbent assay (cf. Section 17.7). The intensity of the emission, is stable during a few minutes, what makes this method extremely sensitive over an extended range of concentrations, which is unusual for this kind of test. 

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