Chemiluminescence detection rearrangement

Before considering detector characteristics and some recent developments in chemiluminescence detection, it should be noted that analytical applications of chemiluminescence involve two types of chemiluminescent response. In the first type, the chemiluminescent molecule is used as a detection label and is, therefore, present in limiting concentration relative to the reagents used to initiate the chemiluminescent reaction. The chemical reaction will therefore be pseudo first order. The slowest process in the sequence of events leading to light emission is the reaction itself, e.g., hydrolysis, bond-breaking, and rearrangements. From Eq. 

In common with all other sensitive detection systems, maintenance of the label enzyme in its active state is important. The precautions detailed in Notes 1—3 should be observed to maximize the sensitivity achieved. Reagents for enhanced chemiluminescence can be prepared in the laboratory or ure available commercially (see Note 4). The purity of the substrate solution is important in achieving maximum sensitivity. Therefore, the precautions detailed in Notes 5-7 should be followed if preparing substrate solutions. The free base form of lummoi undergoes rearrangement ro a mixture of luminol and a series of contaminants. Therefore, luminol should be purified by recrystaliistation as the sodium salt before use (see Note 8). 

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