Chemical proteomics profiling

Heal WP, Wickramasinghe SR, Tate EW (2008) Activity based chemical proteomics profiling proteases as drug targets. Curr Drug Discov Technol 5 200... 

The results for bacterial whole-cell analysis described here establish the utility of MALDI-FTMS for mass spectral analysis of whole-cell bacteria and (potentially) more complex single-celled organisms. The use of MALDI-measured accurate mass values combined with mass defect plots is rapid, accurate, and simpler in sample preparation then conventional liquid chromatographic methods for bacterial lipid analysis. Intact cell MALDI-FTMS bacterial lipid characterization complements the use of proteomics profiling by mass spectrometry because it relies on accurate mass measurements of chemical species that are not subject to posttranslational modification or proteolytic degradation. 

Activity-based protein profiling (ABPP) is a chemical proteomic strategy in which active-site-directed covalent probes are used to profile the functional states of enzymes in complex proteomes. Activity-based probes (ABPs) can distinguish active enzymes from their inactive zymogens or inhibitor-bound forms. They contain a reactive group intended to modify enzyme active sites covalently and a reporter group (typically rhodamine or biotin) that assists in detection and identification of protein targets. 

Wright AT, Cravatt BF (2007) Chemical proteomic probes for profiling cytochrome p450 activities and drug interactions in vivo. Chem Biol 14 1043-1051... 

Rix U, Superti-Furga G (2009) Target profiling of small molecules by chemical proteomics. Nat Chem Biol 5 616-624... 

Neuropeptides, Chemical Activity Profiling and Proteomic Approaches for... 

Screen small molecule libraries in crude protein extracts for inhibitors for comparative chemical proteomics studies (Paulick and Bogyo, 2008) (identifying differences in the expression profile of various enzymes in diseased state and healthy state). 

Figure 1.26 Kinase activity profile of three antileukemia drugs (imatinib, nilo-tinib, and dasatinib) that primarily inhibit the oncogenic BCR-ABL kinase. (From Rix, U. and Superti-Furga, G., 2009, Target Profiling of Small Molecules by Chemical Proteomics, Nat. Chem. Biol, 5(9) 616-24.)...

Rix U et al. (2010) A comprehensive target selectivity sirrvey of the BCR-ABL kinase inhibitor INNO-406 by kinase profiling and chemical proteomics in chronic myeloid leukemia cells INNO-406 target profile in CML. Leukemia 24 44-50... 

Besides protein microarray proteome profiling, the term chemical proteomics (also chemoproteomics or pull-downs ) is mostly used in reference to the application of affinity chromatography protein purification when small molecules are the bait, and liquid chromatography separation of peptides precludes mass spectrometry as the universal readout (LC-MS/MS). 

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