Characterization of Polysaccharides

Polysaccharides with diverse characters are obtained from a variety of plant, animal, and microbial sources. The factors determining the character of a polysaccharide are types 

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Characterization of Polysaccharide Interactions Charuwan Thanawiroon, Wenjun Mao, and Robert J. Linhardt... 

This exaggeration makes possible the characterization of polysaccharides by coordinate orientation (Walter, 1991). For routine purposes, accuracy to more than the squared term is seldom required. 

Gonda, R., Tomoda, M., Shimizu, N. and Kanari, M. (1990) Characterization of polysaccharides having activity on the reticuloendothelial system from the rhizome of C. longa. Chemical and Pharmaceutical Bulletin 38(2), 482-486. 

Polysaccharides can be derivatized by reductive amination at the reducing end group. With highly fluorescing labels like 2-aminoanthracene (2-AA), dex-trans up to a molecular mass of 20 kDa can be visualized and hence analyzed by CE [35]. The separation of the AA derivative (only one chromophore introduced) of a synthetic dextran (4-6 kDa) is shown in Fig. 15. This opens new possibilities in the characterization of polysaccharides. 

We are continuing the characterization of polysaccharide-producing green algae, and the function of the polysaccharides in order to find a way to utilize these metabolites. 

Besides application to the analysis of polysaccharides of unknown structure, enzymes are also often used for routine measurements on polysaccharides of essentially established structure, such as the starch components and the glycogens. Enzymes provide extremely quick and accurate information for the characterization of polysaccharides of this type, including the products synthesized in vitro diuring studies on their mechanism of biosynthesis. There is also increasing application to the characterization of other types of polysaccharide, synthesized enzymically in vitro. A further important use is for investigation of the nature of the products formed by the action of other catabolic enzymes on polysaccharides such applications may give information on either the structure of the polysaccharide or the specificity of the catabolic enzyme under investigation. 

Rioux, L., Turgeon, S. L., and Beaulieu, M. (2007). Characterization of polysaccharides extracted from brown seaweeds. Carbohydr. Polym. 3,530-537. 

Belgacem M.N., Blayo A., Gandini A., Surface characterization of polysaccharides, lignins, printing ink pigments, and ink fillers by inverse gas chromatography, Journal of Colloid and Interface ScL, 182, 1996,431 36. 

Sears KD, Beehk A, Casebier RL, Engen RJ, Hamilton JK, Hergert HL. Southern pine prehydroly-zates characterization of polysaccharides and lignin fragments.J Polym Sci Part C 1971 36 425-43. 

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