Introducing the chromophore

Aromatic, dye and other chromophore-containing molecules have a variety of special physical properties. CDs modified by these groups exhibit many new properties. Since the chromophore can be used as a spectral probe, this makes it possible to apply UV, fluorescence, circular dichroism spectroscopy and other means in the molecular recognition field. For example, CD phosphate has a positive electron deficient center, which is a new recognition site for multiple identification of amino acids. 

Since the fluorophores-modified CDs can be used as the probe and fluorescent sensors for detecting various guest molecules, they have been researched a lot. 

Keep N, N -dicyclohexyl carbodiimide and 4-(l-pyrene)-butyric acid in DMF solution at —4°C. Add 6-deoxy-6-amino-y-CD to the above reaction buffer at room temperature. Then pour the mixture into acetone and recover precipitate. The mono-[6-(l-pyrene)-Ding amido-6-deoxy]-y-CD can be obtained. Finally, the crude products can be purified through eluting by DMF/H2O in the Sephadex LH-20 column. 

The fluorescent CD, N-fluorescein-N -(mono-6-deoxy-6-/3-CD) thiourea, could append to the water-soluble polymer chain to form light-emitting light elements collar, or fluorescence quasi-linear alkyl. 

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The examples mentioned here for RP-HPLC analy sis of monosaccharides in the form of perbenzoates and amino adds as V-phenylthiocarbamoyl derivatives (Table 1) satisfy both prindpal criteria introducing the chromophores into molecules of the analytes... 

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