Cerebellar cortex immunoreactivities

Benagiano, V., Lorusso, L., Coluccia, A., Tarullo, A., Flace, P., Girolamo, F., Bosco, L., Gagiano, R., Amhrosi, G. (2005). Glutamic acid decarboxylase and GABS immunoreactivities in the cerebellar cortex of adult rat after prenatal exposure to a low concentration of carbon monoxide. Neuroscience 135 897-905. 

Electron-microscopical studies indicated that mGluR5 was mainly localized in somatic and dendritic profiles expression of mGluR5 immunoreactivity was observed in the postsynaptic elements in the hippocampus (Lujan et al., 1996, 1997 Hanson and Smith, 1999), basal ganglia (Shigemoto et al., 1993 Hanson and Smith, 1999), thalamus (Godwin et al., 1996 Liu et al., 1998), hypothalamus (Romano et al., 1995 Van den Pol et al., 1995), cerebellar cortex (Negyessy et al., 1997), dorsal cochlear nucleus (Petralia et al., 1996b), and dorsal horn of the spinal cord (Vidnyanszky et al., 1994 Jia et al., 1999). 

Intense mGluR4a immunoreactivity was found in the molecular layer of the cerebellar cortex of the rat this was localized electron-microscopically in axon terminals of the parallel fibers arising from the granule cells in the cerebellar cortex (Kinoshita et al., 1996b Mateos et al., 1999). Presynaptic localization of mGluR4a immunoreactivity was also reported in the other brain regions. In the trapezoid body of the rat, mGluR4a immunoreactivity was observed in... 

Negyessy L, Vidnyanszky Z. Kuhn R, Knbpfel T, Gores TJ, Hamori J (1997) Light and electron microscopic demonstration of mGluR5 metabotropic glutamate receptor immunoreactive neuronal elements in the rat cerebellar cortex. J Comp Neurol 585 641-650. 

Fig. 20. Examples of soraatostatin-immunoreactive elements in the cerebellar cortex of the paraflocculus of adult colchicine treated rats. Patch of Purkinje cells and an immunostained Golgi cell (arrow head) are present in this section. Somatostatin-immunoreactive climbing fibers are observed. Calibration bar 50 jim. Villar et al. (1989).

Fig. 22. Localization of inositol 1,4,5-triphosphate receptor with PCD6 antibody in frozen sections of rat cerebellum by immunofluorescence. Sagittal section of the cerebellar cortex. Small arrows in the granule cell layer (GL) point to segments of immunoreactive axons which represent recurrent collaterals of Purkinje cell axons. Mignery et al. (1989).

Fig. 31. A. Calbindin-D28k immunoreactivity. B. Parvalbumin-immunoreactivity in rat cerebellar cortex. Purkinje cells react with both antibodies arrows in B indicate parvalbumin- immunoreactive stellate and basket cells. Bar = 50 gm. Courtesy of Dr. M.P.A. Schalekamp.

Fig. 54. Ultrastructural localization of mGluR2/3 immunoreactivity in the granular layer of rat cerebellar cortex. Dense immunoreaction products accumulate in axon terminals of Golgi cells, which often make synaptic contacts (curved arrows) with possible granule cell dendrites around a mossy fiber terminal (MT) in the cerebellar glomerulus. Bar = 0.5 fm. Ohishi et al. (1994).

Fig. 75. Photomicrographs of serotonin immunoreactive fibers in selected regions of rat cerebellar cortex. A. Dark-field photomicrograph. B. High-power bright-field photomicrographs illustrating the beaded nature of the immunoreactive fibers (arrows). Vermal lobule X. Abbreviations see Fig. 74. Calibration bar = 200 jum. Bishop and Ho (1985).

The ultrastructure of NA fibers of the cerebellar cortex and other parts of the rat CNS was analyzed with pre-embedding dopamine-y5-hydroxylase immunohistochemistry by Olschowka et al. (1981). Immunoreaction product was present in the axoplasm, associated with smooth endoplasmatic reticulum, Golgi apparatus, synaptic and large dense core vesicles and the outer membranes of mitochondria. Large varicosities were interconnected by narrow intervaricose axon segments. Varicosities, filled with clear, round synaptic vesicles and large dark-core vesicles, made asymmetric contacts with dendrites, but never with somata or axons. More than 50% of the labelled varicosities in the cerebellum made synaptic contacts most of them with dendritic shafts, fewer on spines. 

Ikeda et al. (1991) and DeLacalle et al. (1993) found ChAT immunoreactive neurons in the cerebellar nuclei in cat and man, respectively. According to Ikeda et al. (1991) cholinergic neurons in the cerebellar nuclei project to the cerebellar cortex in the form of mossy fibers, and to the thalamus and the red nucleus (see Section 5.2.). 

Fig. 136. The reconstruction of parasagittal bands of Zebrin I (mabQl 13-immunoreactive) Purkinje cells in the adult rat cerebellar cortex as seen from the anterior (a) and posterior (b). The band pattern is based upon the serial reconstruction of nine complete and five partial cerebella from sections cut in the horizontal plane and four complete reconstructions from sections cut coronally. Bands PI + through P7+ are labelled. Hawkes and Leclerc (1987).

Amenta F, Cavalotta D, Del Valle ME, Mancini M, Sabbatini M, Torres JM, Vega JA (1994) Calbindin D-28k immunoreactivity in the rat cerebellar cortex age-related changes. Neuroscl Lett., 178, 131-134. 

King JS, Ho RH, Bishop GA (1986a) Anatomical evidence for enkephalin immunoreactive climbing fibres in the cerebellar cortex of the opossum. J. Neurocytol, 15, 545-559. 

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