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Centrifugation membrane permeability

In ultrafiltration, water and other low molar mass molecules are forced through a semi-permeable membrane by the application of high pressures (1-7 bar) or of a centrifugal field. This technique involves membranes with pore diameters in the range of 1.0-20 nm, which are most commonly characterized and selected based on their nominal molar mass cut-off... [Pg.305]

Ultrafiltration - Pressure or centrifugation is used to force a mixture of known total concentrations of protein and ligand through a semi-permeable membrane [38]. [Pg.59]

Hydrolysis of sucrose occurs rapidly within the outer portion of the brush-border, plasma membrane, whereupon most of the monosaccharides released are transported across a permeability barrier located in the inner portion of the same membrane. Some hydrolytic products diffuse backward from the mucosal site of formation, and accumulate in the lumen.5-8 The mucosal site of hydrolysis is identified by the use of fluorescent antibodies against sucrase8 and by differential centrifugation of intestinal preparations.2... [Pg.287]

In the second method, ultrafiltration, centrifugal force is applied for a few minutes to a sample of the compound in plasma, pushing the unbound fraction through a permeability selective membrane. LC/MS/MS analysis of the ultrafiltrate and retentate is then done. This has the advantage of being faster than dialysis. Methods like 96-well plate have been developed here too. As in HTS, edge effects have been noted with these, and one study comparing such a method with results from individually run samples for a dozen research compounds showed an value of 0.60. ... [Pg.397]


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Membranes, permeable

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