Cellulose paper chromatogram

Figure 14. Paper chromatogram of the hydrolysis products from higher cellulose substrates by Ex-1. Developed by the descending technique for 96 hr at room temperature on Whatman No. 1 paper, using 1-butanol pyridine water (6 4 3, v/v) as a solvent (S) standard, (Gt) glucose, (Gz) cellobiose, (Gs) cellotriose, (Gu) cellotetraose, (G5) cellopentaose, (G6) cellohexaose final enzyme concentration 2.82 X 10 2%.

Figure 21.12. Comparative TLC and PC separation of nucleotides. A Cellulose thin-layer chromatogram—development distance, 10 cm in 91 min. B Paper chromatogram run under identical conditions—development distance, 10 cm in 134 min paper Schleicher and Schull 20436. The solvent used for both was saturated ammonium sulfatejl M sodium acetate isopropanol (80 18 2). Each vertical column of spots corresponds to separate mixtures separated. Samples (1) 3 -AMP (2) 2 -AMP (3) 3 -GMP (4) I -GMP (5) 2 - and3 -GMP (6) 2 - and3 -UMP (7) 5 -AMP (8) 5 -ADP (9) 5 -ATP. (,A = adenosine, G = guanine, C = cytidine, M = mono-, D = di-, T = tri-, P = phosphate.) From K. Randerath, Biochem. Biophys. Res. Comm., 6, 452 (1961-62), by permission of Academic Press.

The original concept of cellulose as an inert support for the aqueous phase has been modified since the liquid-liquid hypothesis is inadequate for a complete explanation of the process (60,266,267). The mechanism is complex (186,187) and both adsorption and partition probably play a part in the same system. Hanes and Isherwood (122) consider the operation of paper chromatograms to depend upon the relative ease with which solutes pass from the mobile phase into the cellulose-water complex. While its exact nature is controversial, it is thought that cellulose in its hydrated form is a complex gel composed of alternate submicroscopic crystalline and amorph-... 

The use of quantitative, paper chromatography is discussed in this Section, since one of the most important factors in its application to wood-cellulose analysis is that it permits a satisfactory determination of mannan. Reasonably reliable methods for the determination of xylan have been available for some time, but mannan determinations, for the reasons discussed above, have been less satisfactory. The fact that (on chromatograms) xylose, as well as other carbohydrates, can be determined simultaneously with mannose is an added attraction in the use of this technique. 

Yisualisation on cellulose thin-layer chromatograms can be carried out just as in paper chromatography. 

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