CellTracker Green

The 8 C values of cultured B. aculeata are all lower than the 8 C of the DIC in the reservoir water, by amounts ranging from 0.4 to more than 1 Mo. The isotopic offsets are consistently greater for smaller specimens (less than 2 pg per shell, dotted diamonds) than for large specimens (more than 2 p,g per shell, solid diamonds) from the same culture chamber. The 8 C offsets in the culture experiments approach the magnitude of AS C values for live (CellTracker Green, this study (circles)) and Rose Bengal stained (McCorkle et al. (1997) (hexagons)) field specimens. We believe these comparable overall offsets have different explanations pore water microhabitat effects on the field specimens, and carbonate ion concentration effects on the cultured specimens. 

The A8 0 values for cultured B. aculeata are not significantly different from the 8 0 offsets observed in the live (CellTracker Green labeled) B. aculeata field samples from this study, and in the Rose Bengal stained specimens from the North Carolina margin sites of McCorkle et al. (1997) (Fig. 7). The field A8 0 values are calculated as the 8 0 offsets between live (stained) core-top specimens and the 8 0 values predicted for bottom water temperature and 8 0(water). Likewise, the A8 C values in the culture experiments are similar to the... 

Dilute stock CMFDA (CellTracker Green at 10 mM) to 15 jM final concentration in pre-warmed media with no FBS. 

CellTracker orange CMTMR or CellTracker green CMFDA (molecular probes) for cell staining according to section 3.6.2.1, diluted to lOmM final concentration in DMSO (Sigma), 1-pl aliquots, store at -20°C. 

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