Resolution cell

Embertson, J., Zupanic, M., Beneke, T., Till, M., Wolinsky, S., Ribas, J. L., Burke, A., and Haase, A. T. (1993) Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single-cell resolution. Proc. Natl. Acad. Sci. USA 90, 357-361. 

Single-cell resolution Tracks individual live cells and discriminates them from dead cells and extracellular stain allows identification of hormesis and separation of compensatory adaptation from degenerative change allows more accurate identification of sequence of change in different cytotoxicity parameters as cells might not be synchronous or alike in their response... 

Zhang HT, Kacharmina JE, Miyashiro K, Greene MI, Eberwine J. Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution. Proc Natl Acad Sri USA 2001 98(10) 5497-5502. 

Quantitative assays can be performed on large populations, with single cell resolution. Flow cytometric analysis provides the opportunity to examine the distribution of protein functions within a library and to determine the fraction of clones having an activity of interest. 

Fig. 5.2.4. Absorption spectra of alkaline solutions in a CIRCLE cell (resolution 4cm, 32 scans). The spectra are measured against water...

Terrace structure on TiO2(110) (1x1) has been observed with atomic or unit cell resolution by many groups in the past few years [167-180]. There is a general consensus that under conditions of positive sample bias the images are dominated by bright rows running along the [001] direction with a separation of... 

Figure 1. CoefBdent of friction (COF) for various loads. The value presented is the average of the last full turn during the test at a particular load. It is assumed that at the end of the test the COF is representative for the protective film formed under the applied conditions. The error bars are calculated using error propagation from the load-cell resolution. The two samples represent two independent experiments.

Fourier transform of the optical response of a twist-cell to (5-voltage. The OTF dependence on spatial frequency allows us to evaluate the twist cell resolution in spatially nonuniform fields. 

Microchamber-based cell culture is a common 3D cell culture method where cells are cultivated in a microscale chamber structure. This microchamber can easily be fabricated using existing microfabrication technology. It is similar to a miniaturized model of a traditional macroscopic cell culture weU (Fig. 1). Microchambers are used for long-term culture and monitoring of extremely small populations of cells with single-cell resolution. 

Bergner, S., J. Wegener, and F. M. Matysik, Monitoring passive transport of redox mediators across a confluent cell monolayer with single-cell resolution by means of scanning electrochemical microscopy. Anal. Methods, Vol. 4, 2012 pp. 623-629. 

In their work, the authors demonstrated the viability of cells after deposition. As the array was integrated in a microfluidic channel, docked cells could be exposed to chemicals and their responses studied. In these two examples, the authors could analyze the response of a-factor-dependent green fluorescent protein (GFP) expression cells or monitor the response of the mating mitogen-activated protein kinase (MAPK) pathway. These studies could be performed in real time and at the single cell resolution. 

Fig. 3 Visualization of olfactory bulb neurons at single-cell resolution. A whole-mount brain carrying a single-labeled neuron in the olfactory bulb is stained with anti-GFP (a green in b) and anti-SV2 magenta in b) antibodies. Confocal Z-stack images of the forebrain are shown in dorsal view with anterior to the top. OSolfactory bulb, re/telencephalon, rHb right habenula. Scale bar, 30 pm...

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