Cell penetrating peptide sequences

Fig. 13 Left. ELP carrier N-terminally fused to a cell-penetrating peptide and C-terminally fused to a therapeutic peptide. Right Amino acid sequences for several cell-penetrating peptides (see text for details). Reprinted from [83] with permission from Elsevier, cop)mght 2010...

The control of inverse transition temperatures by sequence manipulation and biocompatibility of ELPs make them useful polymers for drug delivery. Cultured cancer cells and solid tumors in animal models uptake fluorescently labeled ELPs in a thermally responsive manner (48,49). Two major limitations in cancer therapy have been the inability of therapeutic molecules to cross the cell membrane and the target-specificity of the compounds. To overcome these limitations cell-penetrating, peptides (CPP) have been fused with ELPs (CPP-ELP) to develop thermally responsive therapeutics with the ability to translocate the cell membrane (Figure 3B). CPPs can assist in the transportation of hydrophilic compounds (small molecules, oglionucleotides and peptides) across the cell membrane (50). Fusing ELPs to a variety of CPPs have revealed that the peptide sequence of penetratin demonstrates the most efficient cellular uptake (51). Further, these CPP-ELPs have been fused to a c-Myc inhibitory peptide known to target and inhibit cancer. As proof of principle, these fusion proteins inhibits proliferation of cultured cancer cell lines in a thermally responsive manner (52). 

TABLE 3.3-1. Examples of Peptide Sequences of Common Cell-Penetrating Peptides... 

Cl. Astriab-Fisher A, Sergueev D, Fisher M, et al. (2002). Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides Effects on cellular uptake, binding to target sequences, and biologic actions. Pharmaceut. Res. 19 744-754. 

Fig. 13 Amino acid sequences of some cell-penetrating peptides (CPP) and translocation of CPP-conjugated carriers across the plasma membrane...

Order, cellular delivery system or component Cell-specific homing sequence Organelle targeting sequence Signal sequence Cell-penetrating vector Delivery vehicle Order, immunological peptide Antigen... 

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