Cell-based screens

Weissman A, Keefer J, Miagkov A, Sathyamoorthy M, Perschke S, Wang FL (2006) Cell-based screening assays. In Taylor JB, Triggle DJ (eds) Comprehensive medicinal chemistry n. Elsevier, Oxford, Sect 3.29... 

Lee JW, Jung M, Rosania GR, Chang YT (2003) Development of novel cell-permeable DNA sensitive dyes using combinatorial synthesis and cell-based screening. Chem Commun 1852-1853... 

Advances in understanding cell-based screening 9. Conclusions References... 

Nakajima M, Ishimuro T, Kato K, Ko IK, Hirata I, Arima Y, Iwata H (2007) Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation. Biomaterials 28 1048-1060... 

Black CB, Duensing TD, Trinkle LS, Dunlay RT. Cell-based screening using high-throughput flow cytometry. Assay Drug Dev. Technol. 2010 Feb 9(l) 13-20. 

Minor, L.K., Westover, L., Kong, Y., Patel, D., Wildey, M.J., Conway, B.R., and Demarest, K.T., Validation of a cell-based screen for insulin receptor modulators by quantification of insulin receptor phosphorylation, /. Biomol. Screen., 8, 439, 2003. 

Hsieh AC, Bo R, Manola J et al (2004) A library of siRNA duplexes targeting the phos-phoinositide 3-kinase pathway determinants of gene silencing for use in cell-based screens. Nucleic Acids Res 32 893-901... 

Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening... 

KeyWords Adenovirus reporter protein luciferase green fluorescent protein high-throughput cell-based screening. 

Here we describe the construction and use of adenoviruses to express lucif-erase- or GFP-fusion proteins for high-throughput cell-based screens. We constructed adenoviruses that express a cyclin Bl-luciferase reporter protein or unmodified luciferase. This enabled us to screen for small molecules that specifically up-regulated levels of the cyclin B 1-luciferase fusion protein without affecting luciferase. A similar approach was used to construct adenoviruses that express cyclin A-GFP or cyclin Bl-GFP fusion proteins. These viruses were used in an imaging-based screen to identify small molecules that specifically upregulated these proteins or altered their subcellular localization. 

Key Words Ras Raf yeast two-hybrid system protein interaction compound library cell-based screening. 

Evans MJ, Saghatelian A, Sorensen EJ et al (2005) Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23 1303-1307... 

We routinely use these substrates for kinetic characterizations of new aldolase catalysts. However, they are not suitable for cell-based screenings because both substrate and product are readily cell permeable. The solution to this problem came when we discovered that our aldolase antibodies catalyze the -elimination (or r ro-Michael reactions) of (3-hetero substituted ketones 37 [Scheme 8 (1)]. 

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