Ablation cells

Belshaw PJ, Spencer DM, Crabtree GR, Schreiber SL. Controlling programmed cell death with a cyclophilin-cyclosporin-based chemical inducer of dimerization. Chem. Biol. 1996 3 731-738. Mallet VO, Mitchell C, Guidotti JE, Jaffray P, Fabre M, Spencer D, Amoult D, Kahn A, Gilgenkrantz H. Conditional cell ablation by tight control of caspase-3 dimerization in transgenic mice. Nat. Biotechnol 2002 20 1234-1239. 

Conditional cell ablation by tight control of caspase-3 dimerization in transgenic mice, Nat. Biotechnol. 2002, 20, 1234-1239. 

In the grasshopper embryo. Ball et al. (1985) have found that, when the motoneurons Df and D turn away from nerve 5 towards their target muscle 133a, their growth cones become closely associated with an identified mesodermal cell, the 133a muscle pioneer, which appears to erect a scaffold for the formation of muscle 133a a mass of mesodermal cells later fuses with this cell. Ablation of the 133a muscle pioneer results in failure of the Df motor axon to leave the nerve 5 pathway at its normal site instead, this axon advances further into the embryonic limb bud (Fig. 13). 

There are various gene therapy approaches that are being developed for the treatment of cancer. They are aimed at inhibiting molecular pathways underlying malignant cell transformation. In other cases, tumor cell ablation by directly applying cell-killing... 

Miller, L., Leor, J., Rubinsky, B., 2005. Cancer cells ablation with irreversible electroporation. Technol. Cancer Res. Treat. 4, 699—705. 

Lin D.M., Auld V.J., and Goodman C.S. 1995. Targeted neuronal cell ablation in the Drosophila embryo Pathfinding by follower growth cones in the absence of pioneers. Neuron 14 707-715. 

Smith H.K., Roberts I.J.H., Allen M.J., Connolly J.B., Moffat K.G., and O Kane C.J. 1996. Inducible ternary control of transgene expression and cell ablation in Drosophila. Dev. Genes Evol. 206 14-24. Spradling A.C. 1986. P-element mediated transformation. In Drosophila A practical approach (ed. D. Roberts), pp. 175-198. IRL Press, Oxford. 

Protocol 25.7 Embryonic Cell Ablation Using Lasers, 473... 

Despite these attractive features, laser methodologies are not without their shortcomings. Cell ablation can be time consuming, especially when multiple cells are ablated in each animal. Genetic approaches will always generate more test animals than laser ablation. In addition, to ablate a cell, it must first be imaged in a live, undissected animal. This often requires video-based microscopy, sometimes with computer enhancement (Halpern et al. 1991 Inoue and Spring 1997). 

Pulsed dye lasers are generally best suited for cell ablation. These devices pump a fluorescent dye to generate an intense output light pulse lasting a few nanoseconds. One advantage of these lasers is that they can be tuned to any wavelength, from the near ultraviolet (UV) to near infrared (IR). Because of their simple construction, they are compact and lightweight, relatively inexpensive, and very reliable. 

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