Elution chromatography cation

Cation elution chromatography is a highly developed separation method in which two or more cations independently compete for complexation between a stationary ion exchanger phase and a mobile solvent phase. The method is most effectively used for... 

Fig. 3. Cation-exchange chromatography of protein standards. Column poly(aspartic acid) Vydac (10 pm), 20 x 0.46 cm. Sample 25 pi containing 12.5 pg of ovalbumin and 25 pg each of the other proteins in the weak buffer. Flow rate 1 ml/min. Weak buffer 0.05 mol/1 potassium phosphate, pH 6.0. Strong buffer same +0.6 mol/1 sodium chloride Elution 80-min linear gradient, 0-100% strong buffer. Peaks a = ovalbumin, b = bacitracin, c = myoglobin, d = chymotrypsinogen A, e = cytochrom C (reduced), / = ribonuclease A, g = cytochrome C (oxidised), h = lysozyme. The cytochrome C peaks were identified by oxidation with potassium ferricyanide and reduction with sodium dithionite [47]...

The dialysed sample was fractionated by cation exchange chromatography. A 40-50 ml sample was applied to a CM-Sepharose CL-6B column (1.5 x 15 cm). Unbound proteins were removed with 50 mM MES pH 6.8, 1 mM DTT, and the bound proteins were eluted with an increasing NaCl gradient from 0 - 0.4 M NaCl in a total volume of 500 ml. The flow was 25 ml/h and fractions of 8.33 ml were collected. The protein profile was measured at 280 nm. 

Purification by cation exchange chromatography (Dowex 50WX8, 200-400 mesh, H" " form) eluted with 1 m NH4OH afforded the amine as a white solid in 78 % yield (48 mg, 0.25 mmol). 

Xylanase II was subjected to cation exchange chromatography with a 20-mm i.d., 700-mm long CM-Trisacryl column eluted with 0.8 mL/min of 0.025M sodium acetate buffer at pH 4.5, with a 0-0.2M sodium chloride gradient. Only one protein peak eluted from the column it contained all the Xylanase n activity. This material appeared... 

The new chemistry is based on a Sr-90/Y-90 separation using a-hydroxyisobutyric acid (a-HIB) and cation exchange chromatography (5). Once the activities are loaded onto the column, the steps to prepare the column for the a-HIB elution remove several of the possibile contaminants including rubidium and cobalt. Finally, the a-HIB elution also removes a wide range of other elements as well, leaving strontium on the ion exchange column (6). 

Figure 3-6 Separation of amino acids by cation-exchange chromatography on a sulfonated polystyrene resin in the Na+ form by the method of Moore and Stein.110 The amino acids were detected by reaction with ninhydrin (Box 3-C) areas under the peaks are proportional to the amounts. Two buffers of successively higher pH are used to elute the amino acids from one column, while a still higher pH buffer is used to separate basic amino acids on a shorter column. From Robyt and White.13...

El 1. Amino acid analyzers are instruments that automatically separate amino acids by cation-exchange chromatography. Predict the order of elution (first to last) for each of the following sets of amino acids at pH = 4. 

Ding, M., Tanaka, K., Hu, W., Hasebe, K., and Haddad, P. R. (2001) Simultaneous Ion-exclusion Chromatography and Cation-exchange Chromatography of Anions and Cations in Environmental Water Samples on a Weakly Acidic Cation-exchange Resin by Elution with Pyridine-2,6-dicarboxylic Acid, Analyst 126, 567-570. 

Cation exchange elution chromatography has been used as our separation method for Am and Cm. The separation conditions have been studied in detail. They involved the dependence of elution solution pH, temperature of the column and resin cross-linking on resolution. When cross-linking of the resin equals 4 per cent and column temperature equals 50° to 75°C, resolution is optimized. 

The first successful separations of rare earths by this technique was achieved fifty years ago. Two techniques used in the separation of rare earths are (i) displacement chromatography and (ii) elution chromatography. Commercial ion exchangers involving both cation exchangers and anion exchangers are listed in Table 1.18. 

A method for the accurate online ultratrace analysis of palladium in environmental samples (road dust and contaminated soil samples) by ICP-MS after separation of interferent cations by cation exchange chromatography was introduced by Hann and co-workers. ° Palladium was selectively adsorbed online onto a Cjg microcolumn which had been reversibly loaded with a complexing agent A,Al-diethyl-Al -benzoylthiourea (DEBT). The palladium complex formed was eluted with methanol and introduced into an ICP-SFMS via microconcentric nebulization with membrane desolvation. Isotope dilution analysis was employed for quantification purposes. ... 

For the sake of completeness a series of Athabasca asphaltene-derived bases was chromatographed using the same column system and solvent sequence for elution and compared with the bases from the resin fraction of the same bitumen. These results are shown in Figure 8. First, the highest MW fraction of the asphaltenes was the tetrahydrofuran/i-propylamine fraction (6500 by VPO). This is one of the fractions that could not be eluted from the column by benzene/methanol/i-propylamine. Apparently, tetrahydrofuran/ t-propylamine is hardly a more polar solution than benzene/methanol/ i-propylamine. In cation exchanger chromatography, the decisive component... 

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