Catecholamine Permeabilized cells

Foran, P., Lawrence, G. W., Shone, C. C., Foster, K. A., and Dolly, J. O. (1996). Botulinum neurotoxin C1 cleaves both syntaxin and SNAR-25 in intact and permeabilized chromaffin cells Correlation with its blockade of catecholamine release. Biochemistry 25, 2630—2636. 

Bittner MA, Holz RW (1988) Effects of tetanus toxin on catecholamine release from intact and digitonin-permeabilized chromaffin cells. In J. Neurochem. 51 451-6... 

Ahnert-Hilger G, Bader MF, BhakDai S et al. (1989b) Introduction of macromolecules into bovine adrenal medullary chromaffin cells and rat pheochromocy-toma cells (PC12) by permeabilization with streptolysin O inhibitory effect of tetanus toxin on catecholamine secretion. J. Neurochem. 52 1751 -8... 

Permeabilized secretory cells are widely used to study the final events during secretion by exocytosis. Convenient cellular models are bovine adrenal chromaffin cells in short term culture and the rat pheochromocytoma cell line PC 12. Both cell types take up labeled catecholamines and store them in secretory vesicles, from which they can be released upon stimulation. The released catecholamines can be detected in the supernatant. After permeabilization of the plasma membrane, release of catecholamines can be triggered by micromolar concentrations of Ca. ... 

Table 1. Catecholamine uptake into permeabilized PC 12 cells... 

Ahnert-Hilger G, Brautigam M, Gratzl M (1987) Ca -stimulated catecholamine release from permeabilized PC 12 cells Biochemical evidence for exocytosis and its modulation by protein kinase C and G-proteins. Biochemistry 26 7842-7848. 

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