Catecholamine Uptake into SLO-Permeabilized PC 12 Cells

Catecholamine uptake is driven by the pH gradient across the vesicle membrane (Schuldiner eta/., 1979). 

Assay of ATP-dependent uptake of catecholamines or amino acid transmitters 

Remove medium from cells, and resuspend them in Krebs-Ringer-HEPES and wash them twice with Krebs-Ringer-HEPES buffer by centrifugation. 

Resuspend pellet in ice cold buffer I containing SLO or alpha-toxin. 

Incubate samples with SLO for 5 min on ice, alpha-toxin-samples for 10 min at 32 °C (incubation time may be increased). 

---