Carotenoid study HPLC analysis

Macula. HPLC analysis was used to study the distribution of carotenoids in the macula (206). The extraction procedure and HPLC analysis of macular carotenoids obtained from human donor eyes on a 5-pm Spherisorb ODS-1 C18... 

Scott, K. et al.. Interlaboratory studies of HPLC procedures for the analysis of carotenoids in foods. Food Chem., 57, 85, 1996. 

Another study employed both TLC and HPLC for the analysis of carotenoids of Calendula officinalis L. TLC separation of all E(trans)-a,3-carotene, cryptoxanthin, zeaxanthin and lutein was performed on a silica layer using petroleum ether-j-propanol-CIICI, (90 10 70 v/v). The same carotenoid pigments were separated by RP-HPLC using an ODS column (250X4 mm, i.d.). The organic modifiers were methanol, THF and ethyl octane. The flow rate was 1 ml/min, pigments were detected at 440 nm [20],... 

Essays in the older hterature (42, 43) deahng with the purification and characterisation of carotenoids have been superseded by recent accounts of the application of HPLC to carotenoid analysis and separation (551) and of modern techniques of high field n.m.r. spectroscopy to elucidation of their structures (216). The mass spectra of several of the fungal carotenoids discussed above have been studied in detail by Liaaen-Jensen (217). 

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