Cardiosphere-derived cells

For fibrous PU scaffolds, the mechanical environment is much more complex than 2D polyacrylamide since the scaffolds are porous, fibers are distributed within the constructs, and cells are attached to single fibers. As a result, the stiffness characteristics for both single fibers and scaffolds may have an impact on stan cell differentiation. We recently studied the effects of PU scaffold stiffness at small and large strains (before and after aUgmnent of fibers, respectively) and single fiber stiffness on cardiac differentiation of cardiosphere-derived cells (CDCs). The single fiber stiffness was... 

Xu Y, Patnaik S, Guo X, Li Z, Lo W, Butler R, et al. Cardiac differentiation of cardiosphere-derived cells in scaffolds mimicking morphology of the cardiac extracellular matrix. Acta Biomater 2014 10 3449-62. 

Cheng, K., Blusztajn, A., Shen, D., Li, T.S., Sun, B., Galang, G., Zarembinski, T.I., Prestwich, G.D., Marban, E., Smith, R.R., Marban, L., 2012. Functional performance of human cardiosphere-derived cells delivered in an in situ polymerizable hyaluronan-gelatin hydrogel. Biomaterials 33, 5317-5324. 

Smith, R.R., Marbdn, E., Marban, L., 2013. Enhancing retention and efficacy of cardiosphere-derived cells administered after myocardial infarction using a hyaluronan-gelatin hydrogel. Biomatter 3, e24490. 

Li, Z.Q., Guo, X.L., Matsushita, S., Guem, J.J. Differentiation of cardiosphere-derived cells into a mature cardiac lineage using biodegradable poly(N-isopropylacrylamide) hydrogels. Biomaterials 32,3220-3232 (2011)... 

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