Carbon monoxide dehydrogenase nickel

Drake HL. S-1 Hu, HG Wood (1980) Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum. J Biol Chem 255 7174-7180. 

Hydrogenases are not the only nickel-containing enzyme, and researchers must therefore compare the maturation of different nickel proteins to obtain an integrated picture of nickel metabolism. Indeed, similarities between some of the hydrogenase-related nickel-processing proteins with urease and carbon monoxide dehydrogenase maturation factors have been noted, and this has facilitated interpretations of the results for... 

Kerby RL, Ludden PW, Roberts GD. 1997. In vivo nickel insertion into the carbon monoxide dehydrogenase of Khodospirillum rubrum molecular and physiological characterization of cooCTJ. J Bacteriol 179 2259-66. 

Nickel is found in at least four enzymes urease, certain hydrogenases, methyl-CoM reductase (in its cofactor F430) of methanogenic bacteria, and carbon monoxide dehydrogenase of acetogenic and methanogenic bacteria 434... 

Nickel in Carbon Monoxide Dehydrogenase from Acetogenic Bacteria 645... 

This is a noncyclic pathway that also results in the fixation of two molecules of C02 to form acetyl-CoA. It was elucidated by Wood, Ljungdahl, Thauer and others as a pathway which is used by acetogenic bacteria to synthesize acetate from C02 in their energy metabolism [21]. The acetyl-CoA pathway resembles the Monsanto process of acetate synthesis from CO and methanol, with one molecule of C02 being reduced to the level of methyltetrahydropterin, while another C02 molecule is reduced to the level of carbon monoxide in the reaction catalyzed by the nickel-dependent carbon monoxide dehydrogenase (Figure 3.3). 

Cramer, S.R, Eidsness, M.K., Pan, W.H., Morton, T.A., Ragsdale, S.W., Dervartanian, D.V, Ljungdahl, L.G. and Scott, R.A. (1987) X-Ray absorption spectroscopic evidence for a unique nickel site in Clostridium thermoaceticum carbon-monoxide dehydrogenase. Inorg. Chem., 26, 2477-2479. 

Smith MC, Barclay JE, Cramer SP, Davies SC, Gu W-W, Hughes DL, Longhurst S, Evans DJ (2002) Nickel-iron-sulfur complexes approaching structural analogues of the active sites of NiFe-hydrogenase and carbon monoxide dehydrogenase/acetyl-CoA synthase. Dalton Trans. 2641-2647... 

Ragsdale, S. W., Clark, J. E., Ljungdahl, L. G., Lundie, L. L., and Drake, H. L., 1983, Properties of purified carbon monoxide dehydrogenase from Clostridium thermoaceticum a nickel, iron-sulfur protein, J. Biol. Chem. 258 2364n2369. 

Since 1975 four classes of nickel metalloenzymes have been identified (77). The nickel hydrogenases and carbon monoxide dehydrogenases are considered here and the dinuclear active site of urease is described in Section IVB. The fourth class, methyl-S-coenzyme-M reductases,... 

Nickel is also found in a number of bacterial carbon monoxide dehydrogenases or CODHs (81). These are all involved in Cj chemistry, they catalyze the oxidation of carbon monoxide to carbon dioxide ... 

As discussed in Section IIIC, it has been suggested that the nickel site in carbon monoxide dehydrogenases is associated with an iron-sulfur cluster 266). Possible geometries include a mixed-metal cubane, [Ni-3Fe-4S] and a sulfur-bridged assembly (Fig. 60). A synthetic [Ni-3Fe-4S] cluster has been characterized and its Mossbauer properties are very similar to those of CODH from C. thermoaceticum 86). 

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