Carbon Lyases

NUCLEOSIDASES AND RELATED ENZYMES - CARBON-CARBON LYASES 

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Immobilization by cross-linking of enzyme crystals appears to be a generic method however, unique protocols must be developed for each individual protein. Preparation of a CLEC form of many types of proteins and classes of enzymes have been reported including hydrolases, oxidoreductases, carbon-carbon lyases and isomerases. Crystallization of the protein is a highly effective purification step, so... 

Enzyme-catalyzed Claisen-type condensations, and metal ion-independent decarboxylases. Carbon-carbon lyases generally involve inversion of configuration in cases where there is no readily identifiable electrophilic component to catalysis. This implies that catalysis of these reactions requires either the concerted or tightly coupled action of two or more active site functional groups, in order to trap (or avoid) unstable intermediates formed along the reaction pathway. 

Elimination reactions. Enzyme-catalyzed elimination reactions )8 to the carbonyl functions of ketones or thioesters, or those involving enamine intermediates, generally proceed by syn processes. anti-Eliminations are generally observed in the remaining cases. As with the carbon-carbon lyases, there appears to be a relationship between reaction stereochemistry and the stability of reaction intermediates. 

Carbon-carbon lyases EC 4.99. Other lyases Carbon-oxygen lyases Carbon-nitrogen lyases Cartxrn-sulfur lyases Carbon-halide lyases... 

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