Carbohydrates sophorose

Figure 11. Polyacrylamide disc gel electrophoretic patterns of extracellular proteins produced by T. reesei QM 9414. The sample applied to the gel on the left was 130 fig extracellular protein from T. reesei my-celia grown on 1% Avicel (29), that applied to the gel on the right was 120 fig extracellular protein produced from sophorose-incubated mycelia. The bands shown here were stained for protein with Coomassie Blue and could, in all cases, also be stained for carbohydrate with the periodic acid-Schiff reagent.

The CBH I (D) is identical in composition and activity to the CBH I (D) previously described (2) from T. reesei QM 9123. The close correspondence of their amino acid contents (Table VI), the nearly identical content of neutral carbohydrate 6.8% by weight for the CBH I (D) produced in the presence of sophorose and 6.7% for T. reesei QM 9123 CBH I (D) grown on cellulose (2), and identical electrophoretic properties clearly argue for a common molecular structure for these CBH s I (D). The CBH II is clearly different from all other CBH s in electrophoretic mobility (Figure 12) and amino acid composition (41), but is devoid of endoglucanase activity and produces predominantly cellobiose (>90% by weight of soluble products) from cellulose. It has a sedimentation coefficient of 3.71 in comparison to CBH I (D), for which a value of 3.66 was obtained. 

T. reesei, a saprophytic fungus, is capable of utilizing a variety of carbohydrates. Yet, only a few carbohydrates induce cellulase production. Inducers include cellulose, cellulose derivatives, lactose, and sophorose (31,32). Mandels and Reese (31,32) studied the inducibility of various sugars and found that sophorose is an excellent cellulase inducer in T. reesei while having little effect in other fungi or bacteria. On further examination they found that trace amounts of sophorose present in glucose caused the apparent ability of glucose to be a cellulase inducer in T. reesei. 

Sophorose, a carbohydrate fonnd in certain types of beans, has the following Haworth structure (13.4, 13.5, 13.6)... 

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