Carbohydrates nondestructive methods

Kiihn ei al. (1959) attempted to remove the carbohydrate from acid-soluble collagen by two nondestructive methods (1) by repeatedly dissolving and reprecipitating the fibers by dialysis, and (2) by repeated extraction of the soluble collagen solution with chloroform whereby the protein separates out at the water/chloroform interface while free carbohydrate remains in the aqueous layer. They confirmed that all the glucosamine and half the hexose could be removed by either method, but the remaining 0.5 % of hexose could not be reduced further by repeated use of either procedure. 

More sensitive, nondestructive methods for determination of selected sugars after/separation are biological tests such as enzymatic reactions (82), and imunochemical detection (83). Imunostaining is particularly popular in molecular biochemistry and clinical chemistry research, due to the possibility of a direct detection of conjugated carbohydrates such as glycolipids (84). Detection of the... 

TLC procedures for, 769-774 Carbenicillin, 448-452 Carbohydrates, 481-505 analytical methods, 481-483 conjugated, 484-485 detection, 495-501 nondestructive methods, 499-501 postchromatographic derivatization, 498-499, 500... 

Much of the research on the l.c. of carbohydrates has focused on analytical, rather than preparative, aspects. In reality, however, the conditions found in the majority of l.c. methods, namely, no sample derivatization, high-resolution separations, and nondestructive detection-techniques, are ideal for the preparation of pure molecules. Thus, most of the analytical l.c. methods previously described can also be used to isolate small quantities of pure compounds. This Section will cover the use of analytical-scale equipment for preparative applications, as well as the use of large-scale and dedicated preparative instruments for this purpose. Prior to discussion of these applications, a general overview of the preparative l.c. of carbohydrates will be given. 

Nicotinic acid (vitamin B3), 1048 Nondestructive detection methods for carbohydrates, 499-501 Nonpolar extraction, 13 Nonpolar steroids, separation of, 974 Normal chamber RPC (N-RPC), 325-326 Nuclear magnetic resonance (NMR), 4,33 Nucleic acids and their derivatives, 921-969 detection general methods, 931-935 reliability, 934 sensitivity, 934... 

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