Carbohydrates mannose-type

Lace et aL proved that testicular hyaluionidase is a glycoprotein of the high-mannose type [70], They removed the carbohydrate moiety of die enzyme using endoglycasidase F and showed that this did not alter the activity and the antigenicity of the enzyme. 

Complex carbohydrates released from glycoproteins were readily profiled by capillary gel electrophoresis with LIF-based detection of 1-aminopyrene-3,6,8-trisulfonic acid (APTS)-labeled sugar molecules (Figure 10) [125]. High-mannose-type oligosaccharides of ribonuclease B were derivatized by APTS and separated by capillary electrophoresis using polyethylene oxide separation medium [126]. 

Glycosylation of the enzyme varies between 0 and 25 %, the main carbohydrates being GlcNAc, Man, Fuc and Xyl. Most of the oligosaccharides correspond to the high-mannose type [22,23] and, in the case of soybean peroxidase, the structure of the major oligosaccharide, which accounts for 60-65% of the total [22], is ... 

T. Ogawa and T. Nukada, Synthesis of a branched mannohexaose, a part structure of a high-mannose-type glycan of a glycoprotein, Carbohydr. Res., 136 (1985) 135-152. 

Figure 11 The natural oligoantennary glycans of cell surface glycoconjugates, such as high-mannose-type glycoproteins, can be simplified by assembly of the biologically relevant saccharide residues on a branched, multivalent non-carbohydrate scaffold molecule affording a so-called multivalent glycomimetic.

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